3D Multiplex Single-Molecule FISH
April 2025
Fluorescence In Situ Hybridization (FISH) is a technique for visualizing individual nucleotides. RNAscope takes this further by detecting multiple single RNA transcripts within cells and tissues at single-cell resolution. While RNAscope has been traditionally used on 2D cell cultures or thin tissue sections, this limits its ability to capture full gene expression profiles. By adapting RNAscope for thicker, 3D tissues, we improve gene expression analysis, target identification, and spatial biology research, offering a more comprehensive view of cellular heterogeneity.