Application Notes
![](https://downloads.microscope.healthcare.nikon.com/production/imager/coverimages/Application-Notes/41927/Mapping-brain-cell-types-with-Cartana_8f52416f9afcc73a2e17f8c24fa98668.jpg)
Mapping brain cell types with CARTANA in situ sequencing on the Nikon Ti2-E microscope
January 2020
In situ sequencing (ISS) kits offered by CARTANA allow scientists to detect transcripts from hundreds of genes directly inside tissue sections with unprecedented throughput and single-cell resolution. The Nikon Ti2-E inverted microscope enables this application by combining its Perfect Focus System 4 focus locking technology and an automated stage with large image scan capabilities. This note demonstrates ISS for mapping transcriptional cell types within the spatial context of mouse brain sections.