Nov 3, 2021
11:00 am EDT / 4:00 pm GMT / 5:00 pm CET
Dr. Laurence Pelletier
Professor, University of Toronto & Lunenfeld-Tanenbaum Research Institute (Sinai Health)
Dr. Kenneth Prehoda
Professor, University of Oregon
Applications Specialist, Yokogawa Life Innovations
Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope. In this webcast, we will explore the concept of optical pixel reassignment and see how it has been applied in cell and developmental biology research. In one example, Dr. Prehoda will describe how live imaging of neural stem cells using both traditional and super-resolution imaging revealed how the plasma membrane plays a role in neurogenesis. In another example, Dr. Pelletier will discuss how the use of “smart” automated low- to super-resolution imaging pipelines can allow us to unravel the mysteries of centrosome biogenesis and function and how the molecular mechanisms underlying their function can go awry in disease states.