Webinars

We are closely monitoring the situation with COVID-19 (coronavirus) and following the guidance of the Centers for Disease Control and Prevention (CDC) and federal, state and local health authorities. Learn more about the measures we have put in place.

 

Stay connected with the latest imaging applications, technologies and relevant topics with Nikon’s live and recorded webinars. These free events are presented by thought leaders in the imaging field and industry professionals. 

Past Webinars

Live imaging of host-pathogen interactions with AI

Speakers:

  • Prof. Pontus Nordenfelt

    Associate Professor at the Infection Medicine (BMC) at Lund University in Lund, Sweden

  • Dr. Simone Lepper

    European Product Manager Imaging Software & High-Content Screening Microscopy

10.30 CEST

Pathogenic bacteria are armed with many mechanisms to colonize and invade human cells. Live fluorescent microscopy is a great tool for studies of these interactions but comes with several limitations in order to achieve a quantitative understanding of the pathogenic processes. Prof. Nordenfelt will describe the work in his group where they are using the AI-based clarify.ai module to enhance the analysis of host cell cytoskeletal dysregulation during host-pathogen interactions.

This webinar is created in conjunction with Bergman Labora.

Image courtesy of Oscar André and Pontus Nordenfelt.

Hosted by:

The axonal cytoskeleton at the nanoscale

Speaker:

  • Christophe Leterrier

    Group Leader NeuroCyto lab, INP CNRS-Aix-Marseille University Marseille, France

The intricate morphology and molecular identity of axons are maintained for decades but also continuously adapt to changes in the environment and activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and mobility of axonal components. In our lab, we use super-resolution microscopy to map the nanoscale architecture actin-based structures within the axon. In the axon initial segment, a key compartment for the maintenance of neuronal polarity, we resolved a highly organized assembly encompassing the periodic actin/spectrin scaffold and its partners: ankyrin, myosin. We have also visualized new actin structures along the axon shaft: rings, hotspots and trails, and are now exploring their molecular organization and functions. For this, we develop a combination of versatile labeling, correlative live-cell/super-resolution/electron microscopy and quantitative analysis that allow for high-content, nanoscale interrogation of the axonal architecture.

Hosted by:

Using the Nikon Ti2 Inverted Platform as an Optical Bench

Speaker:

  • Ian Ross, Ph.D.

    Senior Biosystems Applications Manager, Nikon Instruments Inc.

Flexibility and modularity are core principles underlying Nikon’s imaging systems and components. In this presentation, we will discuss innovative solutions from Nikon that are designed to maximize flexibility and enable easy customization of both home-built microscopes and fully turn-key commercial systems. Learn how the Eclipse Ti2 with its expandable structure, multiple input and output ports, and easy access to the back-aperture plane can be used as an optical bench. Furthermore, Nikon’s wide range of modular illumination devices from TIRF to photo-stimulation can be custom-combined for your unique application. For those requiring full access to the optical path, Nikon also offers the Ti2-Hardware Development Kit for introducing completely custom components. We will also discuss Nikon’s latest objectives utilizing new immersion media and featuring industry-leading numerical aperture and working distance.

Hosted by:

View Recording

Insights into Synaptic Plasticity using STED Microscopy

Speakers:

  • Joachim Fischer, Ph.D.

    Product Manager, Abberior Instruments

  • Dion Dickman, Ph.D.

    Associate Professor of Neurobiology, University of Southern California

In the first half of this webinar, Joachim Fischer, PhD, Product Manager at Abberior will introduce the theory of STED and go over its implementation in the shoebox-sized STEDYCON system. The STEDYCON is a powerful multicolor STED and confocal microscope designed for highest resolution, highly reliable operation and ease of use. It can upgrade existing widefield microscopes to STED microscopes in minutes and provides users with resolution capabilities down to 30nm. In this talk, the optical design of the STEDYCON as well as its imaging capabilities will be highlighted.

In the second half, Dion Dickman, Ph.D., Associate Professor of Neurobiology at USC, will present new insights into synaptic organization and plasticity using STED microscopy. Synapses have the remarkable ability to adaptively modulate synaptic strength in response to perturbations that would otherwise destabilize neurotransmission, referred to as homeostatic synaptic plasticity. Homeostatic signaling systems have emerged as robust and potent regulators of neural activity, enabling stable synaptic function while permitting the flexibility necessary for learning and memory, yet the molecules and mechanisms involved remain poorly understood. Dr. Dickman will first discuss the Drosophila neuromuscular junction as a powerful model system to illuminate fundamental principles of structural and functional synaptic organization and plasticity. He will then present new findings using STED microscopy that reveal active zones, the sites of neurotransmitter secretion at presynaptic terminals, to be substrates for homeostatic modulation.

Hosted by:

View Recording

Defining Functionality in Organoids

Speakers:

  • James Wells

    Chief Scientific Officer, Center for Stem Cell and Organoid Medicine (CuSTOM); Cincinnati Research Foundation Endowed Professor, Division of Developmental Biology; Director for Basic Research, Division of Endocrinology; Allen Foundation Distinguished Investigator

  • Alysson R. Muotri

    Director of the Stem Cell Program; Institute for Genomic Medicine; Dept. of Pediatrics & Cellular Molecular Medicine UCSD School of Medicine UC San Diego

  • Sally Temple

    Scientific Director of the Neural Stem Cell Institute Rensselaer, NY

Moderated by:

  • Claudia Willmes

    Trends in Molecular Medicine

Organoid technology bridges preclinical and clinical science and could solve several research questions and therapeutic challenges. Stem-cell-derived organoids have been established for multiple organs and are used for human disease modeling, host-pathogen interactions, drug discovery and toxicity testing, studying organogenesis, and developing regenerative therapies. Patient-derived organoids and technological advances that allow for targeted manipulation and growing more diverse organoids have added another layer of precision. While this emphasizes their great potential in biomedical applications and precision medicine, the rapid growth of the field also highlights its lack of cohesive definitions. In light of recent scientific breakthroughs and new use cases for organoids, it is only apt that we evaluate what a functional organoid is to exploit its full potential.

Hosted by:

View Recording

Super-resolution microscopy of neuronal proteins

Speaker:

  • Luca Colnaghi, PhD

    Head of Biological Regulation Unit, Mario Negri Institute for Pharmacological Research

Super-resolution microscopy allows us to go beyond the optical limits of traditional microscopes. This technique has been particularly useful in the understanding of the physiology of synapses. In this webinar, we will describe how to study co-localization of proteins with synaptic markers at a super-resolution level. We will discuss the required controls and potential pitfalls and caveats of the approach by describing the co-localization of proteins belonging to the SUMOylation machinery with pre- and post-synaptic markers such as PSD95, synaptophysin and drebrin.

Hosted by:

Track embryo model formation and differentiation of MSC spheroids with Nikon's BioStudio

Speakers:

  • Dr. Erik Vrij

    Postdoctoral Researcher, Maastricht University

  • Dr. Steven Vermeulen

    Postdoctoral Researcher, Maastricht University

  • Dr. Ziryan Othman

    European Product Manager, Nikon Cell Solution Business

We are excited to invite you to this webinar where we are going to discuss the available novel approaches for improving key processes in cell culturing and observation.

  • During the webinar, Nikon will introduce you the most recent cell observation systems (BioStudio-T and BioStudio-mini).
  • Learn more on the generation of MSC spheroids and the analysis of blastoid formation with the help of Nikon’s BioStudio.
  • The systems offer new insight into cellular activity as it provide long-term microscopic observation of live cells and organoids using phase contrast imaging under physiological conditions.
  • The cell observation systems work with Nikons Analysis Software which includes dedicated modules to analyze image sets for cell confluency, human pluripotent stem cell colony growth and differentiation, iPSC reprogramming efficiency, neurite outgrowth, neuronal cell/cluster count, MSC counting and migration and scratch assay.

Hosted by:

Flamingo: Putting Advanced Light Sheet Microscopy in the Hands of Biologists

Speaker:

  • Jan Huisken, Ph.D.

    Director of Medical Engineering, Morgridge Institute for Research, Madison, WI, USA

Light sheet microscopy (SPIM) has changed the field of fluorescence imaging substantially by offering a versatile technique to obtain optical sectioning in large specimens with high speed and minimal phototoxicity. Still, one of the challenges is the customization and dissemination of the technology. In this seminar Jan Huisken will give an introduction to light sheet microscopy, show a few applications in zebrafish and explain the concept and implementation of the Flamingo framework, a modular shareable light sheet microscope.

Hosted by:

View Recording

Neuronal Optical Imaging: From Cellular Landscape to Circuit Functionality

Speakers:

  • Uri Manor, Ph.D.

    Director, Waitt Advanced Biophotonics Core, Salk Institute, USA

  • Valentina Emiliani, Ph.D.

    CNRS Research Director, Wavefront-engineering Microscopy Group, Photonics Department, Institut de la Vision, Inserm, France

  • Xiaowei Zhuang, Ph.D.

    Howard Hughes Medical Institute Investigator; David B. Arnold Jr. Professor of Science; Professor of Chemistry and Chemical Biology; Professor of Physics, Harvard University

  • Rituparna Chakrabarti, Ph.D.

    Scientific Editor, iScience

In recent years, the field of neuronal imaging at the cellular and sub-cellular levels has significantly benefited from the advances in genetic engineering, development of sophisticated high-resolution instrumentation, novel probe development, enhanced computing power, and machine learning algorithms. This Cell Press webinar will showcase how these cutting-edge innovations generate valuable structure-function insight into the neuronal circuit. 

Hosted by:

Advances in Artificial Intelligence in Microscopy

Speakers:

  • Florian Jug, Ph.D

    Research Group Leader, Center for Systems Biology Dresden, Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Fondazione Human Technopole, Milano, Italy

  • Martin Weigert, Ph.D

    Group Leader, EPFL Lausanne, Switzerland

  • Christophe Zimmer, Ph.D

    Research Director, Institut Pasteur, Paris, France

The goal of this webinar is to introduce the audience to deep learning techniques for microscopy image analysis. We will give a short introduction to the foundations of deep learning for image analysis and then cover various methods for image denoising and restorations, for super-resolution microscopy, label-free prediction, and cell/object detection. After the webinar, all speakers will be available for an extensive Q&A, giving the audience the chance to ask technical as well as application specific questions. This webinar is mainly targeted to life-scientists working with microscopy image data.

Hosted by:

View Recording

Organoids as an Infection Model for SARS CoV-2

Speakers:

  • Steeve Boulant, Ph.D.

    Group Leader, Dept. of Infectious Diseases, Virology, Heidelberg University

  • Nuria Montserrat, Ph.D.

    ICREA Research Professor, Pluripotency for Organ Regeneration, Institute for Bioengineering of Catalonia

Organoids are becoming a powerful tool for studying tissue-specific host/pathogen interactions. However, their complex, three-dimensional organization can present major challenges. In this webcast, the speakers will discuss advantages, limitations and pitfalls inherent to organoids for studying host/pathogen interactions, including SARS CoV-2 infection.

Hosted by:

View Recording

Operating an Imaging Core Facility During a Pandemic

Speakers:

  • Julia Fernandez-Rodriguez, Ph.D.

    Head of the Centre for Cellular Imaging, Core Facility, Shalgrenska Academy, Univ. Gothenburg

  • Sebastian Munck, Ph.D.

    Head of the VIB BioImaging Core, VIB Center for Brain & Disease Research, KU Leuven

  • Josh Rappoport, Ph.D.

    Executive Director of Research Infrastructure, Boston College

  • Jennifer Waters, Ph.D.

    Director of Nikon Imaging Center at Harvard Medical School and Chan Zuckerberg Imaging Scientist

Operating a microscopy core facility during the current COVID-19 pandemic poses unique challenges. In this panel discussion, speakers from the US and Europe will present their varying perspectives and experiences to shed light on how core facilities and labs around the world are addressing social distancing and safety concerns for their users as institutions re-open and ramp-up research activities.

Hosted by:

View Recording

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. During this webinar we will cover exciting advances in imaging technology and how these advances are moving microscopy from R&D into screening and quality assurance.

Hosted by:

View Recording

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. With advances in automation, optics, hardware integration, and software, including artificial intelligence, imaging is more powerful, important, and accessible than ever.

Hosted by:

View Recording

On-Demand Virtual Seminars

Nikon is offering virtual seminars on a variety of imaging topics including the latest advances in confocal and super-resolution, how deep-learning can be leveraged to extract more from your data, and high-content screening applications. Fully customized seminars are also available.