Nikon Europe B.V. | Europe & Africa
Artboard 1
fr Change Region

Global Site

AX / AX R with NSPARC

Système de microscope confocal

NIR Imaging Option

Incorporating the NIR Imaging Option enables near-infrared excitation in addition to conventional visible-light excitation. It allows the acquisition of more channels in multicolor fluorescence imaging, contributing to the understanding of complex structures in living specimens.

Télécharger AX / AX R with NSPARC Brochure (15.78MB)

Merged Image


Caractéristiques-clés

Bright, high-definition deep imaging

Near-infrared light has a high penetration depth into tissue and is less affected by light absorption and scattering. Acquiring near-infrared light with a high S/N ratio therefore enables high-definition observation of deep structures within living specimens. In addition, near-infrared light induces less sample autofluorescence which improves imaging clarity, and its low phototoxicity makes it effective for imaging living cells.

DAPI

Alexa Fluor® 488

Alexa Fluor® 568

Alexa Fluor® 750

Multi-stained specimen of HeLa cells acquired with visible and NIR light using the NIR imaging option.
Nucleus (DAPI), Actin (Alexa Fluor® 488), Tubulin (Alexa Fluor® 568), Mitochondria (Alexa Fluor® 750)

High wavelength selection flexibility

NIR imaging extended to the 730 nm and 785 nm lines allows excitation of fluorophores over a wide wavelength range from violet to near-infrared, which is effective for reducing crosstalk between fluorescent channels. Excitation in the NIR allows acquisition of additional dyes and thereby more structural details of specimens in multichannel imaging.

Highly sensitive NIR detection

Conventional GaAsP detectors can be replaced by detectors with high quantum efficiency (QE) in the NIR region, namely an Ex Red GaAsP Unit for 730 nm or a PMT-GAS GaAs Unit for 785 nm, enabling highly efficient NIR dye detection.