Application Notes

Optical microscopy is an invaluable tool for life science research. In each of our application notes we step into a different field to explore how Nikon microscope solutions enable the interrogation of important research questions.

All Application Notes (136)

thumbnail

Quantitative, single-cell analysis of siRNA treatment using smFISH

May 2025

Read More and Download

thumbnail

Feedback microscopy using Nikon AX confocal microscopy and JOBS software to bridge mm to µm structures

May 2025

Read More and Download

thumbnail

Live-Cell Imaging of Plant Fertilization Using Nikon Multiphoton Microscope System

April 2025

Read More and Download

thumbnail

3D Imaging of Kidney Proximal Tubule Microphysiological Systems

March 2025

Read More and Download

thumbnail

Quantitative Live-Cell Imaging to Analyze Immune Cell Secretion Activity and Gene Expression

March 2025

Read More and Download

thumbnail

Speedy and clear 3D imaging of organoids

March 2025

Read More and Download

thumbnail

Imaging both H&E and immunofluorescence staining patterns on a single tissue section using the AX confocal laser microscope

March 2025

Read More and Download

thumbnail

A practical assessment of analytical performances for pseudoislet morphology by GA3 and other image analysis tools

March 2025

Read More and Download

thumbnail

High content screening of lifetime data – A feedback microscopy experiment workflow

February 2025

Read More and Download

thumbnail

Precision Imaging in Complex Tissue Structures

December 2024

Read More and Download

thumbnail

AI-powered “Sample Navigation” simplifies image acquisition! Improved reproducibility and streamlined information sharing

November 2024

Read More and Download

thumbnail

Confocal imaging of immune cells in a vasculature network using the HUMIMIC Chip

November 2024

Read More and Download

thumbnail

3D image analysis reveals drug-induced cellular senescence and increased nucleolus volume: Efficient imaging methods to maximize data acquisition

November 2024

Read More and Download

thumbnail

High-sensitivity confocal microscope detector NSPARC enables live imaging of autophagosomes.

November 2024

Read More and Download

thumbnail

3D Confocal Imaging of Thick Samples Using Z Intensity Correction

October 2024

Read More and Download

thumbnail

3D Super-Resolution Imaging of Glial Cells in Cleared Mouse Brain Tissue using NSPARC

October 2024

Read More and Download

thumbnail

Image-based analysis of intracellular delivery of DNA/RNA therapeutics using NSPARC

September 2024

Read More and Download

thumbnail

Resolving subcellular detail in tissue-mimetic culture

September 2024

Read More and Download

thumbnail

The Ti2-I inverted microscope empowers microinsemination with sophisticated optical performance and operability

June 2024

Read More and Download

thumbnail

The mysterious and resilient creature in the moss around us, the tardigrade - Let's catch it! Let's observe it! -

June 2024

Read More and Download

thumbnail

The mysterious and resilient creature in the moss around us, the tardigrade - on the cutting-edge of research -

May 2024

Read More and Download

thumbnail

Label-free quantitative analysis of cellular senescence and high-content imaging of granules

April 2024

Read More and Download

thumbnail

High-speed, label-free THG imaging of red blood cells using a multiphoton microscope

January 2024

Read More and Download

thumbnail

Long-distance, rapid Ca2+ signaling in plants

January 2024

Read More and Download

thumbnail

Mitochondrial toxicity analysis using the AI-driven, fully automated smart imaging system ECLIPSE Ji

January 2024

Read More and Download

thumbnail

Calculating the percentage of cells with micronuclei using the AI-driven, fully automated smart imaging system ECLIPSE Ji

January 2024

Read More and Download

thumbnail

Analysis of endocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji

January 2024

Read More and Download

thumbnail

Analysis of phagocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji

January 2024

Read More and Download

thumbnail

Digital Staining of Mitochondria using NIS.ai and Their Importance in Drug Evaluation

December 2023

Read More and Download

thumbnail

Visualization of cells cultured on cell-culture inserts and identification of cell regions through AI learning

December 2023

Read More and Download

thumbnail

3D super-resolution imaging of myelin structure in human iPSC-derived myelin sheath organoids by NSPARC

December 2023

Read More and Download

thumbnail

Episcopic brightfield imaging of neuronal cells on high-density microelectrode arrays enables prediction of cell region through AI learning

December 2023

Read More and Download

thumbnail

Automatic isolation of pollen using NIS-Elements General Analysis (GA) and JOBS imaging workflow tools

November 2023

Read More and Download

thumbnail

Protein components of virus particles in SARS-CoV-2-infected cells imaged by N-STORM super-resolution microscope

November 2023

Read More and Download

thumbnail

SMZ25 Research Stereo Microscope for morphological research, taxonomy and type digitisation

November 2023

Read More and Download

thumbnail

Long-term, high-resolution time-lapse imaging of axon self-assembly into motor nerve organoids in a microdevice

November 2023

Read More and Download

thumbnail

A microscopy-based service for cellular signaling pathway analysis

October 2023

Read More and Download

thumbnail

Improvement of ICSI Accuracy by Spindle Visualization

September 2023

Read More and Download

thumbnail

Analysis of nuclear translocation using AI-driven fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Analysis of DNA Damage using AI-driven fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Quantitative analysis of autophagy activity using AI-driven fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Apoptosis assay using AI-driven fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

AI-powered optimization of transfection efficiency by nucleus and cellular region detection from brightfield images

September 2023

Read More and Download

thumbnail

Cell size and morphological analysis is easier and more comfortable with the fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Pre-trained AI-powered label-free detection of cell regions from brightfield images and measurement of fluorescence intensity at the cellular level

September 2023

Read More and Download

thumbnail

Cytotoxicity assay using AI-driven, fully automated ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Label-free cell counting using AI-driven fully automated smart imaging system ECLIPSE Ji

September 2023

Read More and Download

thumbnail

Super-resolution imaging of myelin in mouse brain using a multiphoton confocal microscope

August 2023

Read More and Download

thumbnail

Photostimulation time-lapse imaging of live HeLa cells using confocal microscopy

August 2023

Read More and Download

thumbnail

Diagnostic imaging of mixed connective tissue and epithelial tumors of the mammary gland by label-free imaging using multiphoton microscope

April 2023

Read More and Download

thumbnail

High-resolution macro-to-micro 3D observation of cleared rice anthers : sample preparation in view of the refractive index of immersion media

April 2023

Read More and Download

thumbnail

Confocal Imaging of in vitro BBB model using idenTx platform

April 2023

Read More and Download

thumbnail

High throughput cell proliferation assay with low magnification objective, utilizing high-resolution performance of Digital Sight 50M

March 2023

Read More and Download

thumbnail

Macroscopic and 3D imaging of small flower-like taste buds within the mouth by confocal microscopy　

March 2023

Read More and Download

thumbnail

Confocal imaging of vasculature network using the HUMIMIC Chip

March 2023

Read More and Download

thumbnail

High-content live cell imaging of ROS production in activated macrophages

March 2023

Read More and Download

thumbnail

Automatic spheroid detection and drug efficacy analysis using AX R confocal microscope

March 2023

Read More and Download

thumbnail

Macroscopic target search and high-resolution 3D imaging of gastric afferent nerve endings showing various shapes using a confocal microscope and Denoise.ai

March 2023

Read More and Download

thumbnail

Quantitative Analysis of Mitochondrial Microstructure by Super-Resolution Imaging

March 2023

Read More and Download

thumbnail

Method for selecting a phase-contrast objective for label-free visualization of spatio-temporal dynamics of cell division and organelles

March 2023

Read More and Download

thumbnail

Observation of Cell Culture Inserts Using Episcopic Brightfield Method

February 2023

Read More and Download

thumbnail

Membrane dynamics of recycling endosomes detected by confocal microscopy

January 2023

Read More and Download

thumbnail

High-speed, deep, high-resolution multiphoton imaging using expansion microscopy

December 2022

Read More and Download

thumbnail

Label-Free Morphological Analysis Based on Deep Learning Using Primary Cells

October 2022

Read More and Download

thumbnail

In vivo Deep Imaging of Mouse Brain using Novel Fluoropolymer Nanosheet

October 2022

Read More and Download

thumbnail

Label-free detection of neuron and astrocyte regions using deep learning technology

October 2022

Read More and Download

thumbnail

Mayonnaise observation and label-free analysis of oil droplet particle size distribution using AI

August 2022

Read More and Download

thumbnail

Morphogenesis of stable 3D epithelial sheet domes induced by osmotic gradients, as captured by phase contrast and confocal microscopy

August 2022

Read More and Download

thumbnail

Confocal imaging of 3D biological tissues using the HUMIMIC Chip

June 2022

Read More and Download

thumbnail

A confocal laser scanning microscope sheds light on the contribution of TGN/EE SNARE protein SYP61 and ubiquitin ligase ATL31 to C/N-nutrient stress tolerance in plants

June 2022

Read More and Download

thumbnail

Digital pathology based on intrinsic fluorescence spectra of liver fibrosis using a multiphoton microscope

June 2022

Read More and Download

thumbnail

Reduction of phototoxicity and clarification of fluorescent images by Enhance.ai with high S/N technology using AI

June 2022

Read More and Download

thumbnail

Highly accurate and non-invasive cell counts utilizing machine learning

June 2022

Read More and Download

thumbnail

SHG imaging and quantitative analysis of bone collagen fibers using a multiphoton microscope

June 2022

Read More and Download

thumbnail

High Resolution Confocal Imaging that Accurately Captures Localization of Synapse Region in Cultured Neurons

June 2022

Read More and Download

thumbnail

Macro to micro skeletal muscle fiber formation mechanism captured with large FOV confocal microscope

April 2022

Read More and Download

thumbnail

Digital Staining for Cell-Cycle Analysis through a Combination of Fucci and NIS.ai.

April 2022

Read More and Download

thumbnail

The Effects of Refractive Index Mismatch Between Immersion Liquid and Tissue Clearing Reagent on Image Resolution in Deep Areas

April 2022

Read More and Download

thumbnail

Hepatotoxicity test of drug-induced lipidosis using high-content imaging

March 2022

Read More and Download

thumbnail

Validation of control cells in phenotypic screening and selection of analysis methods according to cell morphology

March 2022

Read More and Download

thumbnail

Cell cycle analysis of keratinocytes and label-free cell counting of mitotic cells using Volume Contrast

March 2022

Read More and Download

thumbnail

Ecology of a fossilized cockroach in amber was revealed by confocal microscopy and thin sectioning technology

February 2022

Read More and Download

thumbnail

Confocal Imaging of CAR-T Cell Dynamics Using an Organ-on-a-chip Platform

February 2022

Read More and Download

thumbnail

Quantitative analysis of lesions of renal foot processes and mitochondria, reusing optical microscopy specimens for clinical renal biopsies, made possible by structured illumination

January 2022

Read More and Download

thumbnail

Automatic morphometric extraction of the rabbit osteocytic lacunae using Segment.ai

December 2021

Read More and Download

thumbnail

Morphogenesis imaging of mCherry-expressing Magnaporthe oryzae transformants with DIC and confocal microscopy

December 2021

Read More and Download

thumbnail

High-speed, high-resolution 3D imaging using resonant scanner and Denoise.ai

November 2021

Read More and Download

thumbnail

High-Definition Imaging of Mouse Neuromuscular Junction Using a Resonant Scanner

November 2021

Read More and Download

thumbnail

Localization analysis of RNA polymerase I using a super-resolution microscope and cluster analysis function

November 2021

Read More and Download

thumbnail

3D enhanced resolution confocal imaging of cortical excitatory mouse neurons uncovers dendritic spinule subsets differing in dynamics, regulation, and function

November 2021

Read More and Download

thumbnail

Low Phototoxicity Long-Term Live Cell Apoptosis Assay Using Label-free Live Cell Imaging

November 2021

Read More and Download

thumbnail

Efficient Confocal Imaging of Dynamics and Changes of Cells utilizing a Large Field of View

November 2021

Read More and Download

thumbnail

In vivo Confocal Imaging of Mouse Organs that Clearly Captures Fast Dynamics

November 2021

Read More and Download

thumbnail

Cell Proliferation Assay and Optimization of HCA Assay, Using Label-Free Live Cell Imaging

September 2021

Read More and Download

thumbnail

The Advantages of Resonant Scanning with Ultra Short Laser Exposure Times in Live Imaging

August 2021

Read More and Download

thumbnail

Development of Technology to Visualize Interaction of Osteoblasts and Osteoclasts in Living Bone Tissue

August 2021

Read More and Download

thumbnail

Super-resolution Imaging of the Nanoscale Architecture of Sertoli Cell Cortical F-actin Network

August 2021

Read More and Download

thumbnail

Wide FOV, High-Resolution Confocal Imaging of Podosomes in Osteoclasts ~ Macro and micro observation ~

July 2021

Read More and Download

thumbnail

Localization Imaging of HORMAD Proteins and Meiotic Cohesins

July 2021

Read More and Download

thumbnail

Selecting the Right Objectives - Bright, Sharp Imaging of Structures down to Deep Areas

June 2021

Read More and Download

thumbnail

3D Imaging of Intestinal Organoid

June 2021

Read More and Download

thumbnail

Quantitative analysis of mitochondrial morphology over time using AI

April 2021

Read More and Download

thumbnail

AI + Macro Imaging

March 2021

Read More and Download

thumbnail

Quantitative 3D Imaging of Living Organs-on-Chips with a High-Speed Point-Scanning Confocal System

January 2021

Read More and Download

thumbnail

Live Imaging of Paneth Cell Secretory Responses in Innate Immunity by Using Three-Dimensional Culture of Small Intestinal Epithelial Cells

January 2021

Read More and Download

thumbnail

Super-resolution imaging of double-stained neurons using DNA-Paint

October 2020

Read More and Download

thumbnail

3D Super-resolution imaging of localization of H3K27me3, Lamin AC and DNA

October 2020

Read More and Download

thumbnail

The Secret Inside Flowers - Imaging Inside Plants Using ClearSee Clearing Reagent

October 2020

Read More and Download

thumbnail

Highly accurate segmentation of cell areas based on DIC images using deep learning

September 2020

Read More and Download

thumbnail

Visualization of microglia-neuron junctions with super-resolution and confocal microscopy

July 2020

Read More and Download

thumbnail

Imaging sodium transients in neurons and astrocytes

May 2020

Read More and Download

thumbnail

Mapping brain cell types with CARTANA in situ sequencing on the Nikon Ti2-E microscope

January 2020

Read More and Download

thumbnail

Nikon NIS-Elements Denoise.ai Software: utilizing deep learning to denoise confocal data

January 2020

Read More and Download

thumbnail

Ti2: Increasing data collection and fidelity by maximizing field of view

September 2019

Read More and Download

thumbnail

Measuring individual hPSC colony areas using hPSC Colony Area Package

June 2019

Read More and Download

thumbnail

A1R HD25: the latest in resonant scanning technology allows new live-cell imaging approaches

February 2019

Read More and Download

thumbnail

Increasing Data Collection and Fidelity by Maximizing Confocal Field of View

November 2018

Read More and Download

thumbnail

Structured Illumination Microscopy (SIM) Imaging Comparison with Confocal

September 2018

Read More and Download

thumbnail

Hardware Triggering: Maximizing Speed and Efficiency for Live Cell Imaging

December 2017

Read More and Download

thumbnail

Application of Patterned Illumination Using a DMD for Optogenetic Control of Signaling

November 2017

Read More and Download

thumbnail

Robotic Microscopy with the Nikon Ti2 for High-Content Analysis Applications

December 2016

Read More and Download

thumbnail

N-STORM with DNA-PAINT for Reliable Multicolor & 3D Single Molecule Localization Imaging

November 2016

Read More and Download

thumbnail

N-SIM for Quantitative Ultra-Structural Analyses of the Nuclear Lamina

October 2016

Read More and Download

thumbnail

Quantitative Cluster Analysis Applications for N-STORM

October 2016

Read More and Download

thumbnail

Nikon’s Large-Format Multiphoton System for Intravital Imaging

December 2015

Read More and Download

thumbnail

Quantitative Analysis Tools and Correlative Imaging Applications for N-STORM

November 2015

Read More and Download

thumbnail

Survival Analysis of Human In Vitro-derived Neurons Using New Live Cell Extended Time-lapse Imaging Technology

June 2015

Read More and Download

thumbnail

Development of an Integrated Bioprocess for Production of NK-92 Cells for Immunotherapy

January 2014

Read More and Download

thumbnail

Antitumor Lymphocyte Kinetic Cytotoxicity Assay (Collaboration with CCRM)

January 2013

Read More and Download

thumbnail

A Non-Invasive Method for Counting Human Pluripotent Stem Cell Numbers by Live Cell Imaging

January 2012

Read More and Download

thumbnail

Screening Assay for PSC Responses to Substrates

January 2011

Read More and Download

thumbnail

Automated Haematopoietic Colony Forming Cell (CFC) Assay

January 2011

Read More and Download

thumbnail

Generation of a Growth Curve for iPS Cells in a Feeder-Free Culture by Non-Invasive Image Analysis

January 2011

Read More and Download

thumbnail

Establishment of an Algorithm for Automated Detection of iPS/non-iPS Cells Under a Culture Condition by Non-Invasive Image Analysis

January 2011

Read More and Download

thumbnail

Automated, Non-Invasive Culture, and Evaluation System for iPS Cells under Neural Differentiation Process

January 2011

Read More and Download

thumbnail

Embryonic Stem Cell Responses to Commercially Available Substrates for Stem Cell Maintenance

January 2011

Read More and Download