Application Notes
Optical microscopy is an invaluable tool for life science research. In each of our application notes we step into a different field to explore how Nikon microscope solutions enable the interrogation of important research questions.
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All Application Notes (136)
Application Note
Feedback microscopy using Nikon AX confocal microscopy and JOBS software to bridge mm to µm structures
May 2025
Application Note
Live-Cell Imaging of Plant Fertilization Using Nikon Multiphoton Microscope System
April 2025
Application Note
Quantitative Live-Cell Imaging to Analyze Immune Cell Secretion Activity and Gene Expression
March 2025
Application Note
Imaging both H&E and immunofluorescence staining patterns on a single tissue section using the AX confocal laser microscope
March 2025
Application Note
A practical assessment of analytical performances for pseudoislet morphology by GA3 and other image analysis tools
March 2025
Application Note
High content screening of lifetime data – A feedback microscopy experiment workflow
February 2025
Application Note
AI-powered “Sample Navigation” simplifies image acquisition! Improved reproducibility and streamlined information sharing
November 2024
Application Note
Confocal imaging of immune cells in a vasculature network using the HUMIMIC Chip
November 2024
Application Note
3D image analysis reveals drug-induced cellular senescence and increased nucleolus volume: Efficient imaging methods to maximize data acquisition
November 2024
Application Note
High-sensitivity confocal microscope detector NSPARC enables live imaging of autophagosomes.
November 2024
Application Note
3D Super-Resolution Imaging of Glial Cells in Cleared Mouse Brain Tissue using NSPARC
October 2024
Application Note
Image-based analysis of intracellular delivery of DNA/RNA therapeutics using NSPARC
September 2024
Application Note
The Ti2-I inverted microscope empowers microinsemination with sophisticated optical performance and operability
June 2024
Application Note
The mysterious and resilient creature in the moss around us, the tardigrade - Let's catch it! Let's observe it! -
June 2024
Application Note
The mysterious and resilient creature in the moss around us, the tardigrade - on the cutting-edge of research -
May 2024
Application Note
Label-free quantitative analysis of cellular senescence and high-content imaging of granules
April 2024
Application Note
High-speed, label-free THG imaging of red blood cells using a multiphoton microscope
January 2024
Application Note
Mitochondrial toxicity analysis using the AI-driven, fully automated smart imaging system ECLIPSE Ji
January 2024
Application Note
Calculating the percentage of cells with micronuclei using the AI-driven, fully automated smart imaging system ECLIPSE Ji
January 2024
Application Note
Analysis of endocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji
January 2024
Application Note
Analysis of phagocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji
January 2024
Application Note
Digital Staining of Mitochondria using NIS.ai and Their Importance in Drug Evaluation
December 2023
Application Note
Visualization of cells cultured on cell-culture inserts and identification of cell regions through AI learning
December 2023
Application Note
3D super-resolution imaging of myelin structure in human iPSC-derived myelin sheath organoids by NSPARC
December 2023
Application Note
Episcopic brightfield imaging of neuronal cells on high-density microelectrode arrays enables prediction of cell region through AI learning
December 2023
Application Note
Automatic isolation of pollen using NIS-Elements General Analysis (GA) and JOBS imaging workflow tools
November 2023
Application Note
Protein components of virus particles in SARS-CoV-2-infected cells imaged by N-STORM super-resolution microscope
November 2023
Application Note
SMZ25 Research Stereo Microscope for morphological research, taxonomy and type digitisation
November 2023
Application Note
Long-term, high-resolution time-lapse imaging of axon self-assembly into motor nerve organoids in a microdevice
November 2023
Application Note
Analysis of nuclear translocation using AI-driven fully automated ECLIPSE Ji
September 2023
Application Note
Quantitative analysis of autophagy activity using AI-driven fully automated ECLIPSE Ji
September 2023
Application Note
AI-powered optimization of transfection efficiency by nucleus and cellular region detection from brightfield images
September 2023
Application Note
Cell size and morphological analysis is easier and more comfortable with the fully automated ECLIPSE Ji
September 2023
Application Note
Pre-trained AI-powered label-free detection of cell regions from brightfield images and measurement of fluorescence intensity at the cellular level
September 2023
Application Note
Label-free cell counting using AI-driven fully automated smart imaging system ECLIPSE Ji
September 2023
Application Note
Super-resolution imaging of myelin in mouse brain using a multiphoton confocal microscope
August 2023
Application Note
Photostimulation time-lapse imaging of live HeLa cells using confocal microscopy
August 2023
Application Note
Diagnostic imaging of mixed connective tissue and epithelial tumors of the mammary gland by label-free imaging using multiphoton microscope
April 2023
Application Note
High-resolution macro-to-micro 3D observation of cleared rice anthers : sample preparation in view of the refractive index of immersion media
April 2023
Application Note
High throughput cell proliferation assay with low magnification objective, utilizing high-resolution performance of Digital Sight 50M
March 2023
Application Note
Macroscopic and 3D imaging of small flower-like taste buds within the mouth by confocal microscopy
March 2023
Application Note
High-content live cell imaging of ROS production in activated macrophages
March 2023
Application Note
Automatic spheroid detection and drug efficacy analysis using AX R confocal microscope
March 2023
Application Note
Macroscopic target search and high-resolution 3D imaging of gastric afferent nerve endings showing various shapes using a confocal microscope and Denoise.ai
March 2023
Application Note
Quantitative Analysis of Mitochondrial Microstructure by Super-Resolution Imaging
March 2023
Application Note
Method for selecting a phase-contrast objective for label-free visualization of spatio-temporal dynamics of cell division and organelles
March 2023
Application Note
Observation of Cell Culture Inserts Using Episcopic Brightfield Method
February 2023
Application Note
Membrane dynamics of recycling endosomes detected by confocal microscopy
January 2023
Application Note
High-speed, deep, high-resolution multiphoton imaging using expansion microscopy
December 2022
Application Note
Label-Free Morphological Analysis Based on Deep Learning Using Primary Cells
October 2022
Application Note
In vivo Deep Imaging of Mouse Brain using Novel Fluoropolymer Nanosheet
October 2022
Application Note
Label-free detection of neuron and astrocyte regions using deep learning technology
October 2022
Application Note
Mayonnaise observation and label-free analysis of oil droplet particle size distribution using AI
August 2022
Application Note
Morphogenesis of stable 3D epithelial sheet domes induced by osmotic gradients, as captured by phase contrast and confocal microscopy
August 2022
Application Note
A confocal laser scanning microscope sheds light on the contribution of TGN/EE SNARE protein SYP61 and ubiquitin ligase ATL31 to C/N-nutrient stress tolerance in plants
June 2022
Application Note
Digital pathology based on intrinsic fluorescence spectra of liver fibrosis using a multiphoton microscope
June 2022
Application Note
Reduction of phototoxicity and clarification of fluorescent images by Enhance.ai with high S/N technology using AI
June 2022
Application Note
SHG imaging and quantitative analysis of bone collagen fibers using a multiphoton microscope
June 2022
Application Note
High Resolution Confocal Imaging that Accurately Captures Localization of Synapse Region in Cultured Neurons
June 2022
Application Note
Macro to micro skeletal muscle fiber formation mechanism captured with large FOV confocal microscope
April 2022
Application Note
Digital Staining for Cell-Cycle Analysis through a Combination of Fucci and NIS.ai.
April 2022
Application Note
The Effects of Refractive Index Mismatch Between Immersion Liquid and Tissue Clearing Reagent on Image Resolution in Deep Areas
April 2022
Application Note
Hepatotoxicity test of drug-induced lipidosis using high-content imaging
March 2022
Application Note
Validation of control cells in phenotypic screening and selection of analysis methods according to cell morphology
March 2022
Application Note
Cell cycle analysis of keratinocytes and label-free cell counting of mitotic cells using Volume Contrast
March 2022
Application Note
Ecology of a fossilized cockroach in amber was revealed by confocal microscopy and thin sectioning technology
February 2022
Application Note
Confocal Imaging of CAR-T Cell Dynamics Using an Organ-on-a-chip Platform
February 2022
Application Note
Quantitative analysis of lesions of renal foot processes and mitochondria, reusing optical microscopy specimens for clinical renal biopsies, made possible by structured illumination
January 2022
Application Note
Automatic morphometric extraction of the rabbit osteocytic lacunae using Segment.ai
December 2021
Application Note
Morphogenesis imaging of mCherry-expressing Magnaporthe oryzae transformants with DIC and confocal microscopy
December 2021
Application Note
High-speed, high-resolution 3D imaging using resonant scanner and Denoise.ai
November 2021
Application Note
High-Definition Imaging of Mouse Neuromuscular Junction Using a Resonant Scanner
November 2021
Application Note
Localization analysis of RNA polymerase I using a super-resolution microscope and cluster analysis function
November 2021
Application Note
3D enhanced resolution confocal imaging of cortical excitatory mouse neurons uncovers dendritic spinule subsets differing in dynamics, regulation, and function
November 2021
Application Note
Low Phototoxicity Long-Term Live Cell Apoptosis Assay Using Label-free Live Cell Imaging
November 2021
Application Note
Efficient Confocal Imaging of Dynamics and Changes of Cells utilizing a Large Field of View
November 2021
Application Note
In vivo Confocal Imaging of Mouse Organs that Clearly Captures Fast Dynamics
November 2021
Application Note
Cell Proliferation Assay and Optimization of HCA Assay, Using Label-Free Live Cell Imaging
September 2021
Application Note
The Advantages of Resonant Scanning with Ultra Short Laser Exposure Times in Live Imaging
August 2021
Application Note
Development of Technology to Visualize Interaction of Osteoblasts and Osteoclasts in Living Bone Tissue
August 2021
Application Note
Super-resolution Imaging of the Nanoscale Architecture of Sertoli Cell Cortical F-actin Network
August 2021
Application Note
Wide FOV, High-Resolution Confocal Imaging of Podosomes in Osteoclasts ~ Macro and micro observation ~
July 2021
Application Note
Selecting the Right Objectives - Bright, Sharp Imaging of Structures down to Deep Areas
June 2021
Application Note
Quantitative 3D Imaging of Living Organs-on-Chips with a High-Speed Point-Scanning Confocal System
January 2021
Application Note
Live Imaging of Paneth Cell Secretory Responses in Innate Immunity by Using Three-Dimensional Culture of Small Intestinal Epithelial Cells
January 2021
Application Note
3D Super-resolution imaging of localization of H3K27me3, Lamin AC and DNA
October 2020
Application Note
The Secret Inside Flowers - Imaging Inside Plants Using ClearSee Clearing Reagent
October 2020
Application Note
Highly accurate segmentation of cell areas based on DIC images using deep learning
September 2020
Application Note
Visualization of microglia-neuron junctions with super-resolution and confocal microscopy
July 2020
Application Note
Mapping brain cell types with CARTANA in situ sequencing on the Nikon Ti2-E microscope
January 2020
Application Note
Nikon NIS-Elements Denoise.ai Software: utilizing deep learning to denoise confocal data
January 2020
Application Note
Ti2: Increasing data collection and fidelity by maximizing field of view
September 2019
Application Note
A1R HD25: the latest in resonant scanning technology allows new live-cell imaging approaches
February 2019
Application Note
Increasing Data Collection and Fidelity by Maximizing Confocal Field of View
November 2018
Application Note
Structured Illumination Microscopy (SIM) Imaging Comparison with Confocal
September 2018
Application Note
Hardware Triggering: Maximizing Speed and Efficiency for Live Cell Imaging
December 2017
Application Note
Application of Patterned Illumination Using a DMD for Optogenetic Control of Signaling
November 2017
Application Note
Robotic Microscopy with the Nikon Ti2 for High-Content Analysis Applications
December 2016
Application Note
N-STORM with DNA-PAINT for Reliable Multicolor & 3D Single Molecule Localization Imaging
November 2016
Application Note
N-SIM for Quantitative Ultra-Structural Analyses of the Nuclear Lamina
October 2016
Application Note
Quantitative Analysis Tools and Correlative Imaging Applications for N-STORM
November 2015
Application Note
Survival Analysis of Human In Vitro-derived Neurons Using New Live Cell Extended Time-lapse Imaging Technology
June 2015
Application Note
Development of an Integrated Bioprocess for Production of NK-92 Cells for Immunotherapy
January 2014
Application Note
Antitumor Lymphocyte Kinetic Cytotoxicity Assay (Collaboration with CCRM)
January 2013
Application Note
A Non-Invasive Method for Counting Human Pluripotent Stem Cell Numbers by Live Cell Imaging
January 2012
Application Note
Generation of a Growth Curve for iPS Cells in a Feeder-Free Culture by Non-Invasive Image Analysis
January 2011
Application Note
Establishment of an Algorithm for Automated Detection of iPS/non-iPS Cells Under a Culture Condition by Non-Invasive Image Analysis
January 2011
Application Note
Automated, Non-Invasive Culture, and Evaluation System for iPS Cells under Neural Differentiation Process
January 2011
Application Note
Embryonic Stem Cell Responses to Commercially Available Substrates for Stem Cell Maintenance
January 2011