Nikon Instruments Introduces New Silicone Immersion Objective Lens for Super-Resolution and Multi-Modal Imaging
May 22, 2017
CFI SR HP Plan Apochromat Lambda S 100XC Sil objective and silicone immersion medium enable superresolution imaging of thick specimens and offers correlative imaging capabilities
Nikon Instruments Inc. today unveiled a new silicone immersion objective lens, CFI SR HP Plan Apochromat Lambda S 100XC Sil, for super-resolution and multi-modal imaging. The new silicone immersion objective lens from Nikon provides high numerical aperture and long working distance to enable super-resolution imaging of thick samples as well as correlative imaging with conventional applications such as confocal. Nikon Instruments will unveil the CFI SR HP Plan Apochromat Lambda S 100XC Sil at the 17th International European Light Microscopy Initiative Meeting, May 23-26 in Dubrovnik, Croatia.
“As super-resolution imaging techniques such as SIM and STORM grow in popularity, there are increasing demands to adapt these applications to thicker samples and to correlate data acquired with different super-resolution and conventional techniques,” said Steve Ross, Ph.D., General Manager, Products and Marketing, Nikon Instruments Inc. “We’re pleased to introduce an objective lens to meet those needs.”
CFI SR HP Plan Apochromat Lambda S 100XC Sil Features
- Enables High resolution Imaging of Thick Specimens - The CFI SR HP Plan Apochromat Lambda S 100XC Sil objective is designed for use with Nikon’s new silicone oil to provide high resolution imaging of thick samples. Nikon’s newly developed silicone oil closely matches the refractive index of the specimen to reduce spherical aberration when imaging through thick samples, resulting in bright and clear images. It also achieves excellent viscosity to maintain the immersion liquid during long-term observation and time-lapse imaging.
- Ideal for super-resolution microscopy "N-SIM" "N-STORM" - The new silicone immersion objective is compatible with both structured illumination (N-SIM) and single-molecule localization (N-STORM) based super-resolution techniques. It is highly corrected for axial chromatic aberration and supports high laser power transmission. Combined with its high N.A. and long working distance, the objective enables 3D, multi-color super-resolution imaging of thick samples.
- Achieves accurate chromatic aberration reduction and high transmission rates across a broad spectrum, from UV to near IR - Chromatic aberration is precision-corrected over a wide wavelength range from visible to ultraviolet. Nikon’s highly acclaimed, proprietary, anti-reflective Nano Crystal Coat is utilized to achieve high transmission from UV to near IR wavelengths.
- Improves operability for multi-imaging modalities - The new silicone immersion objective is compatible with confocal systems to enable correlative imaging between confocal and super-resolution techniques. It also eliminates the need to switch objective lenses between N-SIM and N-STORM, providing a seamless workflow between different super-resolution techniques.
|Model name||CFI SR HP Plan Apochromat Lambda S 100XC Sil|
|Working distance||0.3 mm|
|Parfocal distance||60 mm|
|Usable cover glass thickness||0.15 - 0.19 mm|
|Usable temperature||23 to 37°C /73.4 to 98.6° F|
The CFI SR HP Plan Apochromat Lambda S 100XC Sil objective is now available. Visit https://www.nikoninstruments.com/cfi-sr-hp-apo