Notes d'application
Analysis of endocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji
janvier 2024
Endocytosis is a fundamental cellular process in which extracellular materials are taken up by cells and utilized or degraded. It is also a known route of infection for small pathogens and viruses. Observing endocytosis provides useful insights on cellular responses to a wide variety of substances such as drug candidates or pathogens. The ECLIPSE Ji digital inverted microscope equipped with NIS-Elements Smart Experiment (SE) software enables automated, seamless imaging workflows from acquisition to analysis and data visualization. This application note introduces the Endocytosis SE module, which was used to quantify the endocytic activity of A431 cells by measuring the fluorescence intensity and distribution of fluorescently labeled dextran.