Notes d’application

Super-resolution imaging of double-stained neurons using DNA-Paint

octobre 2020

Dr. Noriko Koganezawa (Life Science), Department of Neurobiology and Behavior, Graduate School of Medicine, Gunma University, focuses her research on drebrin, an actin-binding protein that is thought to play an important role in cognitive function. Drebrin accumulates in the posterior synaptic region (dendritic spine) of mature neurons, but when synaptic dysfunction occurs, this accumulation disappears. Also, when the NMDA receptor is activated by glutamate or the like, the accumulation of drebrin is transiently eliminated (Figs.1 and 2). Focusing on the dynamics of drebrin, Dr. Koganezawa is conducting research to verify its effects on cognitive function.

In this Application Note, we introduce examples of image acquisition of changes in the localization of drebrin by glutamate stimulation using the N-STORM super-resolution microscope and quantitative evaluation by cluster analysis.