Webinaires

Stay connected with the latest imaging applications, technologies and relevant topics with Nikon’s live and recorded webinars. These free events are presented by thought leaders in the imaging field and industry professionals.

Past Webinars

Defining Physiological Normoxia in Cell Physiology for Improved Clinical Translation

Speakers:

  • Giovanni E. Mann

    Professor, King's College London, United Kingdom

  • Krista Rantanen

    Director of Scientific Applications, Baker Ruskinn

Oxygen levels in cell research: how important is this? Can it affect the outcome? Join our discussion and learn more about physiological normoxia and how this can affect the translation of findings to interventions

Prof. Giovanni E. Mann will discuss the control of environmental factors including ambient oxygen, CO2, pH, and the composition of cell culture media and why this is critical to ensure physiologically relevant cellular function. This lecture focuses on the importance of conducting cell culture experiments under physiological oxygen levels encountered by different cells types in vivo, with the aim of enhancing the translation of in vitro findings into in vivo scenarios (see Keeley & Mann, Physiological Reviews 2019; 99: 161–234).

Successively, Dr. Krista Rantanen, Director of Scientific Applications @Baker Ruskinn, will discuss the solutions that can help researchers maintain a stable environment for their cells.

Presented by:

AX Confocal – Optimizing Detection, Improving Performance

Speaker:

  • Adam White, Ph.D., Nikon Instruments Inc.

    Advanced Microscopy Product Manager, Nikon Instruments Inc.

With the design of the AX confocal, Nikon focused on the little things. We believed that the sum of these improvements would deliver a significant leap in instrument performance. In this presentation, a few of these optimizations will be described in detail with a focus on how this redesign might change the way we use a point-scanning confocal.

Presented by:

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Automated high content imaging and analysis for biomedical research applications

Speakers:

  • Dr. Eric Griffis

    Director, Nikon Imaging Center at UC San Diego

  • Dr. Amit R. Majithia

    Assistant Professor of Endocrinology, UC San Diego School of Medicine

High content imaging and analysis is a useful tool for applications in biomedical research, such as drug discovery. This webcast will highlight advances in high content imaging capabilities and how they can be integrated into new research studies.

Dr. Eric Griffis, Director of The Nikon Imaging Center at University of California San Diego will provide a technical overview of this technology, including key considerations for its use.

Next, Dr. Amit Majithia of UC San Diego School of Medicine will discuss the utility of high content imaging for studying Non-alcoholic fatty liver disease (NAFLD), the most common chronic liver disease, which affects a quarter of the global adult population. NAFLD can progress to non-alcoholic steatohepatitis (NASH) which is one of the leading causes of liver failure. There are no FDA approved medications to treat NAFLD. Recent large-scale human genetic association studies have identified genes that alter susceptibility to NAFLD in people, but how these genes work in the liver and which ones could be beneficial to target remains unknown. To address these challenges, the Majithia lab has established a platform for genetic perturbation and high content imaging of cellular readouts in human liver cell models scalable to studying hundreds of genes simultaneously.

Learn:

  • Capabilities of a modern high content imaging and analysis system
  • Research and development applications now enabled by advances in high content imaging
  • How high content imaging can help pioneer new treatments for currently intractable conditions

Presented by:

Applying AI to Cell Imaging

Speakers:

  • Jeffery B. Bylund, Ph.D.

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments Inc.

  • Meredith Calvert, Ph.D.

    Principal Scientist/Lab Leader—Microscopy & Image Analysis, Denali Therapeutics

  • Chee Yeun Chung, Ph.D.

    Scientific Co-founder and Senior Director, Yumanity Therapeutics

Moderated by:

  • Karen Tkach Tuzman, Ph.D.

    Senior Editor, Head of Discovery & Preclinical Development, BioCentury

New algorithms and optical system improvements are expanding the frontier of cell imaging quality. Artificial intelligence techniques enable better visualization of cellular mechanisms in a wide number of applications and across multiple scientific disciplines, including advancing neurological research. This webinar will highlight how AI and imaging are being used to improve our understanding of complex and notoriously intractable disorders such as Alzheimer’s, Dementia, and related diseases. Interact with the following imaging and therapeutic development experts to ask them your own questions during the session.

Presented by:

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Super-resolution spinning disk confocal microscopy for life science research

Speakers:

  • Dr. Laurence Pelletier

    Professor, University of Toronto & Lunenfeld-Tanenbaum Research Institute (Sinai Health)

  • Dr. Kenneth Prehoda

    Professor, University of Oregon

  • Dan Collins

    Applications Specialist, Yokogawa Life Innovations

Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope. In this webcast, we will explore the concept of optical pixel reassignment and see how it has been applied in cell and developmental biology research. In one example, Dr. Prehoda will describe how live imaging of neural stem cells using both traditional and super-resolution imaging revealed how the plasma membrane plays a role in neurogenesis. In another example, Dr. Pelletier will discuss how the use of “smart” automated low- to super-resolution imaging pipelines can allow us to unravel the mysteries of centrosome biogenesis and function and how the molecular mechanisms underlying their function can go awry in disease states.

Presented by:

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Higher and Better Content Imaging - Leveraging Advancements in Technology Towards Improved Applications for Imaging in Drug Discovery, Development, and Other R&D Applications

Speakers:

  • Jeff Bylund, Ph.D.

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments Inc.

  • Niranj Chandrasekaran, Ph.D.

    Senior Postdoctoral Associate, Broad Institute of MIT and Harvard University

In the first half of the webinar, we will focus on acquiring and processing good imaging data sets. This will include a discussion of recent advances in hardware and software technology that are making automated imaging workflows more accessible to researchers. The expanding scope of possible imaging applications (3D, organs-on-chips, cell paint, etc.) and turn-key automation options bring opportunities for nearly any research group to improve their workflow. We will also discuss the important role that AI plays in this exciting field.

In the second half of the webinar, we focus on maximizing imaging data sets using advanced analysis methods. Dr. Chandrasekaran (Broad Institute) will discuss how image-based profiling can transform drug discovery. Image-based profiling can be used to mine for relevant patterns, often revealing unexpected biological activity that is useful for many steps in the drug discovery process. Such applications include identifying disease-associated, screen-able phenotypes, understanding disease mechanisms and predicting a drug's activity or mechanism of action. Several of these applications have been recently validated and have moved into production mode within academia and the pharmaceutical industry. Additionally, novel computational technologies such as deep learning that better leverage image-based information hold promise for accelerating drug discovery.

This event will be hosted by the Nikon BioImaging Lab @ SmartLabs. Located in Cambridge, Massachusetts, the Nikon BioImaging Lab is a state-of-the-art imaging facility offering contract research services for cell-based assays.

Presented by:

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Intervention of AI and machine learning for optical imaging

Speakers:

  • Anna Kreshuk

    Group Leader, Cell Biology and Biophysics Unit, European Molecular Biology Laboratory

  • Ricardo Henriques

    Group Leader of the Optical Cell Biology Laboratory, Instituto Gulbenkian de Ciência; Honorary Chair of Computational and Optical Biophysics University College London

  • Rituparna Chakrabarti

    Scientific Editor, iScience

Moderated by:

  • Sarah Callaghan

    Patterns, Editor-in-Chief

Improvements in the technologies associated with optical imaging have meant that there is an increasing amount of better quality, finer resolution data made available for research and diagnosis. The increase in the total volume of data makes it more time consuming for the experts to view all the images from a scan, so training an artificial intelligence system to be able to quickly view images and flag potential abnormalities for review by an expert is of great value.

In this webinar we hear from Anna Kreshuk (European Molecular Biology Laboratory) and Ricardo Henriques (Instituto Gulbenkian de Ciência) who will present on super-resolution microscopy, and how AI and machine learning can assist in developing our understanding of nanoscale biological processes, including viral infection.

Presented by:

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How to create AI-Based Image Analysis Protocols for Cell Culture Samples in Neuroscience

Speakers:

  • David Akbar

    Imaging Engineer at the ICMQuant imaging core facility in the Brain Institute (ICM) in Paris, France

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

How can we develop and automate analysis protocols with AI?

How can we prepare and execute the training phase for Deep Learning?

How can we apply those in the field of Neuroscience?

Welcome to our webinar series "Unlocking next-level research with AI" where we discuss how Artificial Intelligence and deep learning can help researchers understand in-vivo processes faster and accelerate their findings.

David Akbar, Imaging Engineer at the ICMQuant imaging core facility in the Paris Brain Institute (ICM), will discuss his work in developing and automating analysis protocols and applications with three General Analysis (GA) and Artificial Intelligence (AI) tools. Moreover, he will give examples in simplifying the characterization and morphological analysis of neurons as well as colocalization and spot detection.

Successively, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Presented by:

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How to Image and Quantify Human Astrocytes in Alzheimer's Disease Chimeras with AI

Speakers:

  • Dr. Amaia Arranz

    Achucarro Basque Center for Neuroscience and Ikerbasque, Spain

  • Mrs. Nikky Corthout

    Light Microscopy and Imaging Network LiMoNe - Dr. Sebastian Munck. VIB BioImaging Core Leuven - Center for Brain and Disease Research

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Dr. Amaia Arranz studies Alzheimer's disease using murine xenograft models of human neurons and glial cells exposed to disease-associated factors. Dr. Arranz aims to unravel human-specific pathways and mechanisms involved in the pathogenesis of Alzheimer´s and other neurodegenerative diseases to aid their diagnosis and treatment. In her talk, Dr. Arranz will present her research and elucidate the use of Denoise.ai in astrocyte quantification.

Mrs. Nikky Corthout is an expert microscopist dedicated to assisting researchers at the core facility of the VIB Center for brain and disease research focusing on neurobiology and brain disease. Nikky is experienced with imaging of a wide variety of samples ranging from neuronal cultures and brain sections, to entirely cleared brains.

Presented by:

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How to revolutionize our microscope image analysis with AI

Speakers:

  • Dr. Anne-Sophie Mace

    Image Analysis Expert at the Cell and Tissue Imaging Facility at Curie Institute in Paris, France

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

How can we revolutionize our microscope image analysis?

How can we automate the extraction of quantitative parameters from our images?

Can we create a tailor-fit workflow for different imaging needs?

Dr. Anne-Sophie Mace will discuss how she manages to automate the extraction of quantitative parameters such as shape descriptors, co-localization, movements, characteristics, and others.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Presented by:

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Quantification methods in neurodegenerative diseases

Speakers:

  • Prof. Clemens Kaminski

    Department of Chemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, United Kingdom

  • Dr. Lies De Groef

    Neural Circuit Development and Regeneration Research Group, Division of Animal Physiology and Neurobiology, Department of Biology, Katholiek Universiteit Leuven, in Leuven, Belgium.

  • Dr. Adam Glaser

    Department of Mechanical Engineering, University of Washington, Seattle, United States of America

  • Dr. Sebastian Munck (Webinar Host)

    VIB BioImaging Core, VIB Center for Brain & Disease Research, KU Leuven, Department of Neurosciences in Leuven, Belgium.

Register and follow the debate between key opinion leaders in neuroimaging, where they will talk about the future of quantitative methods in neurodegenerative diseases. The speakers will discuss how to use superresolution microscopy to understand diseases, how retinal models teach us about neurodegeneration, and how light-sheet microscopy and optical clearing reveal tissue composition. In addition, you will have the opportunity to ask your questions at the live Q&A session. This webinar is organized by the Nikon Center of Excellence at Katholieke Universiteit (KU) in Leuven, Belgium, and Nikon Europe BV in Amsterdam the Netherlands.

This webinar is organized by the Nikon Center of Excellence at Katholieke Universiteit (KU) in Leuven, Belgium, and Nikon Europe BV in Amsterdam the Netherlands.

Presented by:

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How to perform quantitative pathology with AI

Speakers:

  • Dr. Marko Popovic

    Technical director Microscopy and Cytometry Core Facility, Amsterdam UMC location VUmc

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Is there an improvement when we apply machine learning algorithms at the center of this workflow?

Dr. Marko Popovic will discuss how combining machine learning and data analysis helps perform quantitative pathology and assess the efficacy of different cancer treatments in patients.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Recovering contrast, improving the signal-to-noise ratio, managing challenging acquisition parameters or segmentation have been challenging parts of the image analysis. You can now seamlessly automate these parts with the support of AI and accelerate your research.

Artificial Intelligence and deep learning are powerful tools that help researchers to understand processes in-vivo faster.

Presented by:

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How to build a Bioimage Analysis Workflow with multiple image analysis tools

Speakers:

  • Dr. Carlo Antonio Beretta

    Imaging Specialist, Dept. Anatomy and Cell Biology & Institute for Pharmacology, Heidelberg University

  • Dr. Simone Lepper

    European Product Manager, Imaging Software & Screening, Nikon Europe BV

Join our AI webinar series and learn how researchers from different fields use artificial intelligence to accelerate image analysis.

How can we investigate biological processes with lower phototoxicity in-vivo?

What can we obtain from imaging them at high temporal resolution?

Can we create a workflow that combines deep learning with standard machine learning?

Artificial Intelligence and deep learning are powerful tools that help researchers to understand processes in-vivo faster.

Dr. Carlo Antonio Beretta, from the Department for Anatomy and Cell Biology and Institute of Pharmacology at Heidelberg University, will discuss how deep learning assisted him in tackling significant obstacles that emerged while observing brain tumor invasion.

Moreover, he will discuss a workflow to accelerate the generation of precise manual ground truth data for automated 3D nuclei segmentation.

In turn, Dr. Simone Lepper, European Product Manager Imaging Software & High-Content Screening Microscopy, will discuss how Artificial Intelligence (AI) and deep learning methods are making seemingly impossible tasks now possible.

Recovering contrast, improving the signal-to-noise ratio, managing challenging acquisition parameters or segmentation have been challenging parts of the image analysis. You can now seamlessly automate these parts with the support of AI and accelerate your research.

Presented by:

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Live imaging of host-pathogen interactions with AI

Speakers:

  • Prof. Pontus Nordenfelt

    Associate Professor at the Infection Medicine (BMC) at Lund University in Lund, Sweden

  • Dr. Simone Lepper

    European Product Manager Imaging Software & High-Content Screening microscopy

10.30 CEST

Pathogenic bacteria are armed with many mechanisms to colonize and invade human cells. Live fluorescent microscopy is a great tool for studies of these interactions but comes with several limitations in order to achieve a quantitative understanding of the pathogenic processes. Prof. Nordenfelt will describe the work in his group where they are using the AI-based clarify.ai module to enhance the analysis of host cell cytoskeletal dysregulation during host-pathogen interactions.

This webinar is created in conjunction with Bergman Labora.

Image courtesy of Oscar André and Pontus Nordenfelt.

Presented by:

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The axonal cytoskeleton at the nanoscale

Speaker:

  • Christophe Leterrier

    Group Leader NeuroCyto lab, INP CNRS-Aix-Marseille University Marseille, France

The intricate morphology and molecular identity of axons are maintained for decades but also continuously adapt to changes in the environment and activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and mobility of axonal components. In our lab, we use super-resolution microscopy to map the nanoscale architecture actin-based structures within the axon. In the axon initial segment, a key compartment for the maintenance of neuronal polarity, we resolved a highly organized assembly encompassing the periodic actin/spectrin scaffold and its partners: ankyrin, myosin. We have also visualized new actin structures along the axon shaft: rings, hotspots and trails, and are now exploring their molecular organization and functions. For this, we develop a combination of versatile labeling, correlative live-cell/super-resolution/electron microscopy and quantitative analysis that allow for high-content, nanoscale interrogation of the axonal architecture.

Presented by:

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Insights into Synaptic Plasticity using STED Microscopy

Speakers:

  • Joachim Fischer, Ph.D.

    Product Manager, Abberior Instruments

  • Dion Dickman, Ph.D.

    Associate Professor of Neurobiology, University of Southern California

In the first half of this webinar, Joachim Fischer, PhD, Product Manager at Abberior will introduce the theory of STED and go over its implementation in the shoebox-sized STEDYCON system. The STEDYCON is a powerful multicolor STED and confocal microscope designed for highest resolution, highly reliable operation and ease of use. It can upgrade existing widefield microscopes to STED microscopes in minutes and provides users with resolution capabilities down to 30nm. In this talk, the optical design of the STEDYCON as well as its imaging capabilities will be highlighted.

In the second half, Dion Dickman, Ph.D., Associate Professor of Neurobiology at USC, will present new insights into synaptic organization and plasticity using STED microscopy. Synapses have the remarkable ability to adaptively modulate synaptic strength in response to perturbations that would otherwise destabilize neurotransmission, referred to as homeostatic synaptic plasticity. Homeostatic signaling systems have emerged as robust and potent regulators of neural activity, enabling stable synaptic function while permitting the flexibility necessary for learning and memory, yet the molecules and mechanisms involved remain poorly understood. Dr. Dickman will first discuss the Drosophila neuromuscular junction as a powerful model system to illuminate fundamental principles of structural and functional synaptic organization and plasticity. He will then present new findings using STED microscopy that reveal active zones, the sites of neurotransmitter secretion at presynaptic terminals, to be substrates for homeostatic modulation.

Presented by:

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Defining Functionality in Organoids

Speakers:

  • James Wells

    Chief Scientific Officer, Center for Stem Cell and Organoid Medicine (CuSTOM); Cincinnati Research Foundation Endowed Professor, Division of Developmental Biology; Director for Basic Research, Division of Endocrinology; Allen Foundation Distinguished Investigator

  • Alysson R. Muotri

    Director of the Stem Cell Program; Institute for Genomic Medicine; Dept. of Pediatrics & Cellular Molecular Medicine UCSD School of Medicine UC San Diego

  • Sally Temple

    Scientific Director of the Neural Stem Cell Institute Rensselaer, NY

Moderated by:

  • Claudia Willmes

    Trends in Molecular Medicine

Organoid technology bridges preclinical and clinical science and could solve several research questions and therapeutic challenges. Stem-cell-derived organoids have been established for multiple organs and are used for human disease modeling, host-pathogen interactions, drug discovery and toxicity testing, studying organogenesis, and developing regenerative therapies. Patient-derived organoids and technological advances that allow for targeted manipulation and growing more diverse organoids have added another layer of precision. While this emphasizes their great potential in biomedical applications and precision medicine, the rapid growth of the field also highlights its lack of cohesive definitions. In light of recent scientific breakthroughs and new use cases for organoids, it is only apt that we evaluate what a functional organoid is to exploit its full potential.

Presented by:

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Super-resolution microscopy of neuronal proteins

Speaker:

  • Luca Colnaghi, PhD

    Head of Biological Regulation Unit, Mario Negri Institute for Pharmacological Research

Super-resolution microscopy allows us to go beyond the optical limits of traditional microscopes. This technique has been particularly useful in the understanding of the physiology of synapses. In this webinar, we will describe how to study co-localization of proteins with synaptic markers at a super-resolution level. We will discuss the required controls and potential pitfalls and caveats of the approach by describing the co-localization of proteins belonging to the SUMOylation machinery with pre- and post-synaptic markers such as PSD95, synaptophysin and drebrin.

Presented by:

Track embryo model formation and differentiation of MSC spheroids with Nikon's BioStudio

Speakers:

  • Dr. Erik Vrij

    Postdoctoral Researcher, Maastricht University

  • Dr. Steven Vermeulen

    Postdoctoral Researcher, Maastricht University

  • Dr. Ziryan Othman

    European Product Manager, Nikon Cell Solution Business

We are excited to invite you to this webinar where we are going to discuss the available novel approaches for improving key processes in cell culturing and observation.

  • During the webinar, Nikon will introduce you the most recent cell observation systems (BioStudio-T and BioStudio-mini).
  • Learn more on the generation of MSC spheroids and the analysis of blastoid formation with the help of Nikon’s BioStudio.
  • The systems offer new insight into cellular activity as it provide long-term microscopic observation of live cells and organoids using phase contrast imaging under physiological conditions.
  • The cell observation systems work with Nikons Analysis Software which includes dedicated modules to analyze image sets for cell confluency, human pluripotent stem cell colony growth and differentiation, iPSC reprogramming efficiency, neurite outgrowth, neuronal cell/cluster count, MSC counting and migration and scratch assay.

Presented by:

Flamingo: Putting advanced light sheet microscopy in the hands of biologists

Speaker:

  • Jan Huisken, Ph.D.

    Director of Medical Engineering

Light sheet microscopy (SPIM) has changed the field of fluorescence imaging substantially by offering a versatile technique to obtain optical sectioning in large specimens with high speed and minimal phototoxicity. Still, one of the challenges is the customization and dissemination of the technology. In this seminar Jan Huisken will give an introduction to light sheet microscopy, show a few applications in zebrafish and explain the concept and implementation of the Flamingo framework, a modular shareable light sheet microscope.

Presented by:

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Neuronal Optical Imaging: From Cellular Landscape to Circuit Functionality

Speakers:

  • Uri Manor, Ph.D.

    Director, Waitt Advanced Biophotonics Core, Salk Institute, USA

  • Valentina Emiliani, Ph.D.

    CNRS Research Director, Wavefront-engineering Microscopy Group, Photonics Department, Institut de la Vision, Inserm, France

  • Xiaowei Zhuang, Ph.D.

    Howard Hughes Medical Institute Investigator; David B. Arnold Jr. Professor of Science; Professor of Chemistry and Chemical Biology; Professor of Physics, Harvard University

  • Rituparna Chakrabarti, Ph.D.

    Scientific Editor, iScience

In recent years, the field of neuronal imaging at the cellular and sub-cellular levels has significantly benefited from the advances in genetic engineering, development of sophisticated high-resolution instrumentation, novel probe development, enhanced computing power, and machine learning algorithms. This Cell Press webinar will showcase how these cutting-edge innovations generate valuable structure-function insight into the neuronal circuit. 

Presented by:

Advances in Artificial Intelligence in Microscopy

Speakers:

  • Florian Jug, Ph.D

    Research Group Leader, Center for Systems Biology Dresden, Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; Fondazione Human Technopole, Milano, Italy

  • Martin Weigert, Ph.D

    Group Leader, EPFL Lausanne, Switzerland

  • Christophe Zimmer, Ph.D

    Research Director, Institut Pasteur, Paris, France

The goal of this webinar is to introduce the audience to deep learning techniques for microscopy image analysis. We will give a short introduction to the foundations of deep learning for image analysis and then cover various methods for image denoising and restorations, for super-resolution microscopy, label-free prediction, and cell/object detection. After the webinar, all speakers will be available for an extensive Q&A, giving the audience the chance to ask technical as well as application specific questions. This webinar is mainly targeted to life-scientists working with microscopy image data.

Presented by:

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Organoids as an Infection Model for SARS CoV-2

Speakers:

  • Steeve Boulant, Ph.D.

    Group Leader, Dept. of Infectious Diseases, Virology, Heidelberg University

  • Nuria Montserrat, Ph.D.

    ICREA Research Professor, Pluripotency for Organ Regeneration, Institute for Bioengineering of Catalonia

Organoids are becoming a powerful tool for studying tissue-specific host/pathogen interactions. However, their complex, three-dimensional organization can present major challenges. In this webcast, the speakers will discuss advantages, limitations and pitfalls inherent to organoids for studying host/pathogen interactions, including SARS CoV-2 infection.

Presented by:

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Operating an Imaging Core Facility During a Pandemic

Speakers:

  • Julia Fernandez-Rodriguez, Ph.D.

    Head of the Centre for Cellular Imaging, Core Facility, Shalgrenska Academy, Univ. Gothenburg

  • Sebastian Munck, Ph.D.

    Head of the VIB BioImaging Core, VIB Center for Brain & Disease Research, KU Leuven

  • Josh Rappoport, Ph.D.

    Executive Director of Research Infrastructure, Boston College

  • Jennifer Waters, Ph.D.

    Director of Nikon Imaging Center at Harvard Medical School and Chan Zuckerberg Imaging Scientist

Operating a microscopy core facility during the current COVID-19 pandemic poses unique challenges. In this panel discussion, speakers from the US and Europe will present their varying perspectives and experiences to shed light on how core facilities and labs around the world are addressing social distancing and safety concerns for their users as institutions re-open and ramp-up research activities.

Presented by:

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Microscope Incubators for Live Cell Imaging

Speaker:

  • Lucs Lanzaro, Ph.D

    CEO of Okolab

Which type of microscope incubator can better fit my needs: a cage incubator enclosing the whole microscope or a heated stage top chamber? Is Long-term Live Cell Imaging possible with any of these microscopy techniques: light-sheet, SIM, Super-resolution, reflected light, multiphoton, TIRF ? How can I control Oxygen levels (over that temperature, CO2 and humidity) during time-lapse experiments? Is any microscope incubator compatible with perfusion and microfluidic devices? Join the webinar to find out answers to these and more Live Cell Imaging related questions.

After registering, you will receive a confirmation email containing information about joining the webinar.

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. During this webinar we will cover exciting advances in imaging technology and how these advances are moving microscopy from R&D into screening and quality assurance.

Presented by:

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An extraordinary microscope: expect the unexpected!

Speaker:

  • Alberto Diaspro, Ph.D.

    Department of Physics, University of Genoa, Italy Nanoscopy, Italian Institute of Technology, Italy

The meaning is related to series of advances and new methods that allow getting information at the nanoscale or preferable at the molecular scale referring to both spatial resolution or structural information.The optical microscope, from two-photon excitation to superesolved fluoresnce microscopy, becomes an extraordinary microscope. It has has tunable and flexible performances depending on the biological question and reflects the integration in a flexible way of several approaches. It is worth noting that new correlative approaches coupling optical super resolved methods with scanning probe microscopes and Mueller matrix microscopy are providing interesting developments that will be outlined. Considering all these aspects we can re-phrase the sentence related to the Galilei's "occhialino" into" Microscopium extraordinarium nominare libuit".

The hidden world revealed: in vivo study of Ca2+ dynamics in plants

Speaker:

  • Alex Costa, Ph.D.

    Dipartimento di Bioscienze, Università degli Studi Milano

Older technologies (e.g. aequorin) enabled detection of cytosolic Ca2+signatures triggered by different stimuli at low spatial resolutions. Recent developments in microscopy techniques coupled with the use of plants expressing genetically encoded fluorescent Ca2+ sensors are now revealing a hidden world where Ca2+ increases not only occur in individual, stimulated cells but propagates as waves within the plant body. The use of specific targeting sequences or tissue-specific promoters for sensor expression, is also enabling investigation into Ca2+dynamics in different subcellular compartments and in different tissues.

In the webinar, the technical aspects of the imaging tools needed to perform Ca2+ imaging in plants will be presented.

Driving cells with light-controlled topographies

Speakers:

  • Alberto Puliafito, Ph.D

    Department of Oncology, University of Torino, 10043 Orbassano, Torino, Italy. Candiolo Cancer Institute FPO-IRCCS, 10060 Candiolo, Torino, Italy.

  • Prof. Emiliano Descrovi, Ph.D.

    Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino. Department of Electronic Systems, Norwegian University of Science and Technology – NTNU, N-7491 Trondheim, Norway

In this webinar we will show how to mechanically stimulate cells adhering on a substrate. We will focus on the use of a widefield microscope to both deform the substrate where cells are growing and to monitor cell changes over time. Particular attention will be devoted to quantitative image analysis needed to characterize cell response upon mechanical stimuli. An overview of image analysis techniques such as segmentation and tracking will be presented.

Imaging Applications for R&D, Screening & Quality Assurance

Speaker:

  • Jeff Bylund, Ph.D

    Applications Manager, Stem Cell & Regenerative Medicine, Nikon Instruments, Inc.

Collecting imaging data on the microscopic scale has become an essential part of any life sciences venture. Images not only provide striking visual insights into the microscopic world, they also contain a host of quantifiable, multi-dimensional information that can be used to improve key analytical processes in multiple areas of the biotech and pharma workflow. With advances in automation, optics, hardware integration, and software, including artificial intelligence, imaging is more powerful, important, and accessible than ever.

Presented by:

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