Notes d’application

Localization Imaging of HORMAD Proteins and Meiotic Cohesins

juillet 2021

During meiotic prophase I homologous chromosomes undergo pairing/synapsis and paternal and maternal chromosomes exchange genetic information. It is known that HORMAD proteins (HORMAD1 and HORMAD2) localize along the unsynapsed chromosome axes at that time, and that they monitor homolog synapsis. However, the molecular mechanism by which HORMAD proteins, which do not contain a definitive DNA-binding domain, localize to the chromosomal axes is still unclear. This application note introduces the study that clarified the mechanism by which HORMAD1 localizes to chromosome axes, and its interactions with the meiotic cohesins RAD21L and REC8, this study being the result of joint research conducted by Dr. Yasuhiro FUJIWARA of the Laboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, and Dr. Kei-ichiro ISHIGURO of the Institute of Molecular Embryology and Genetics, Kumamoto University.