High numerical aperture, long working distance lenses for multiphoton imaging and electrophysiology applications
CFI75 LWD 16X W
The CFI75 LWD 16X W is a water dipping objective lens with low magnification, high NA, and long working distance. This lens, when combined with Nikon's ECLIPSE FN1 microscope and dedicated magnification module, additionally provides 5.6x, 32x, and 64x magnifications. The lens is ideal for patch-clamp experiments due to the ease of switching between a large field of view for searching and a smaller field of view for high-resolution visualization. With excellent IR transmission, this lens is also suitable for IR-DIC observation. The objective's high NA provides superb image quality in combination with confocal laser microscopes.
In addition, the 2 mm field of view (magnification 5.6x) and wide 45° approach angle make manipulator control and positioning easy.
CFI75 Apochromat 25XC W
This lens provides a high numerical aperture of 1.10 while still maintaining a long working distance of 2.0 mm. Chromatic aberration is well-corrected up to the near-infrared range. This lens features an adjustable ring to correct for depth-dependent spherical aberration. Together with its 33° manipulator/pipette access angle, it is ideal for both deep imaging of live specimens using multi-photon excitation and physiology research applications.
Download Objectives Brochure (8.74MB)
Specifications
| Model | Dimensions | Transmittance | NA | W.D.
(mm) | Cover glass thickness | Correction ring | Observation |
|---|
| CFI75 Apochromat LWD 20XC W | Diagram | Graph | 1.00 | 2.80 | 0 | ∨ | BF, FL (visible light, NIR) |
| CFI75 Apochromat 25XC W | Diagram | Graph | 1.10 | 2.00 | 0 | ∨ | BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV) |
| CFI75 Apochromat 25XC W 1300 | Diagram | Graph | 1.10 | 2.00 | 0 | ∨ | BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV) |
| CFI75 LWD 16X W | Diagram | Graph | 0.80 | 3.00 | 0 | | BF, DF (Oil), DIC, NIR DIC, POL, FL (visible light, UV) |
BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence
*Possible but not recommended
**External phase contrast observation is possible with ECLIPSE Ti2-E
