Figure 3: Maintaining image quality during fast acquisition with NSPARC. (A) Depth-coded 3D view of cells proliferating off the edge of the block. The 221 x 221 x 151 μm volume was imaged in resonant scanning mode using the 40x Plan Apo λS silicone immersion objective and NSPARC detector. In total, 151, 1024 px frames were collected over 76 seconds. The cutaway reveals the plane and region of interest for panels B and C. Solid grey line indicates underlying Bio-Block structure. (B, C) Comparison of cells imaged beneath 75 μm of combined media and cell mass using (B) the traditional confocal detector in galvo scanning mode with 4096 x 4096 pixel resolution versus (C) the NSPARC detector in resonant scanning mode with 1024 x 1024 pixel resolution.