Objective Comparison

CFI Apo
TIRF 100XC Oil		 CFI S Plan Fluor
ELWD 20XC
Material Number MRD01991 MRH08230
Type Apochromat Super Plan Fluor
Primary Technique TIRF Brightfield
Immersion Oil Air
Magnification 100X 20X
Numerical Aperture 1.49 0.45
Working Distance 0.16–0.09 8.2–6.9
Cover Glass Thickness 0.13–0.19 0–2
CFI Apo
TIRF 100XC Oil		 CFI S Plan Fluor
ELWD 20XC
Diagram
(mm)
Transmittance Graph
CFI Apo
TIRF 100XC Oil		 CFI S Plan Fluor
ELWD 20XC
Brightfield recommended for best results recommended for best results
Darkfield   universal condenser (dry) and darkfield ring, darkfield condenser (dry/oil)
DIC suitable suitable
Fluorescence (visible) recommended for best results recommended for best results
Fluorescence (UV) possible with visible light that has a longer wavelength than the excitation light used for DAPI recommended for best results
Fluorescence (NIR)    
Polarizing suitable suitable
Polarizing Type Simple POL Simple POL
Phase Contrast Ring EXT PH4  
Phase Contrast Type
CFI Apo
TIRF 100XC Oil		 CFI S Plan Fluor
ELWD 20XC
Correction Collar ✓ ✓
Spring Loaded
Iris    
Long Working Distance   ✓
Ti2-E PFS Compatible ✓ ✓
CFI Apo
TIRF 100XC Oil		 CFI S Plan Fluor
ELWD 20XC
Asbestos    
Clear Tissue    
Clinical / Laboratory    
Confocal ✓  
Education    
Laser Trapping / Tweezing ✓  
Multiphoton    
SIM ✓  
STORM ✓  
Research ✓ ✓
TIRF ✓  
Tissue Culture   ✓