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AACR 2025 – Multiomics Spatial Analysis of Colon Cancer Tissue Reveals Emergence of an Immunosuppressive Tumor Maintenance System
avril 2025
Using a combination of modalities such as sequential multiplex immunofluorescence (mIF) and spatial transcriptomics (STx) on colon cancer sample we investigated spatial distribution, phenotype, function, and gene expression profile within the colon cancer and healthy tissue. Immunosuppressive cells, Treg and M2 macrophages were in the TME. Presence of other immune cells was not sufficient to prevent crypt hyperproliferation. While immunosuppressive phenotype was observed using antibodies, a strong presence of immunosuppressive genes was not found in transcriptomic study. Analysis of this multi-omics dataset showed hypoxia, a known factor to drive immunosuppression and COL6A2 gene expression to have strong association in driving tumor progression.