Notes d’application

3D Super-resolution imaging of localization of H3K27me3, Lamin AC and DNA

octobre 2020

In this Application Note, we introduce examples of imaging (using the N-SIM super-resolution microscope) performed by pharmaceutical scientist Dr. Tsukasa Oikawa of the Department of Molecular Biology, Hokkaido University Graduate School of Medicine. In the absence of p53, the product of a tumor suppressor gene TP53, K27-trimethylation of histone H3 (H3K27me3) was observed to occur at the perinuclear regions during DNA replication. The researcher then wanted to know the positional relationship between H3K27me3, the nuclear membrane represented by Lamin A/C, and DNA.