ECLIPSE Ni Series

Stratum structure capable of mounting multiple optical paths

• Ni-E
• Ni-L

Nikon's proprietary stratum structure enables simultaneous mounting of two optical paths on one microscope. This structure allows vertical attachment and individual control of an excitation filter cube turret and a barrier filter cube turret, enabling support for a variety of applications.

Focusing mechanisms can be modified for the application

• Ni-E

Ni-E's focusing mechanism can be selected from focusing stage type and focusing nosepiece type. The focusing nosepiece type enables a fixed-stage experimental system and meets the needs of in vivo imaging.

Confocal imaging

• Ni-E

Combining the Ni-E’s high-precision Z-focus mechanism with Nikon’s confocal microscope system allows high-resolution, high-S/N-ratio imaging of 3D structures of organs and cells. The Ni-E has a highly stable structure suitable for mounting a confocal scanner and features a wide luminous flux that enables bright confocal imaging.

Learn more about AX / AX R Confocal Microscope System

Simultaneous multichannel imaging

• Ni-E
• Ni-L

The Ni’s flexible stratum structure allows the back camera port unit and the epi-fluorescence attachment to be mounted at the same time, enabling simultaneous image acquisition of two different wavelengths with each camera. This enables the capture of high-resolution images in the entire frame for each wavelength without dividing the CCD chip. The use of individual cameras for acquisition allows the user to tailor acquisition parameters for each channel independently, allowing acquisition of high-sensitivity FRET images.

* For information about compatible cameras, contact Nikon or Nikon dealers.

Imaging of fluorescent-labeled specimens

• Ni-E
• Ni-L

In combination with a motorized nosepiece and a motorized epi-fluorescence cube turret, each unit is automatically controlled according to the camera settings such as exposure time, camera gain, and time interval, allowing fast, efficient image acquisition.

The motorized epi-fluorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated with a convenient Ni-L remote control pad.

Automated imaging of pathological specimens

• Ni-E

With the Ni-E, optimal brightness can be automatically adjusted with objective changeover, eliminating the need for manual adjustment. By controlling the optical zooming of the motorized DSC zooming port for quadrocular tube, it is possible to capture images with the desired angle of field while maintaining the image quality.

Automatic adjustment with objective changeover

• Ni-E

Condenser, aperture, field diaphragm and ND filter are automatically set to optimal positions according to the objective magnification in use. In addition, XYZ stage travel distance per handle rotation and parfocal distance deviation correction are automatically adjusted. Microscope settings can also be manually adjusted.

Reproducing observation conditions

• Ni-E

Selected observation conditions can be designated to individual buttons, enabling changes to be made at the push of a button. This is particularly convenient when reproducing specific observation conditions.

High-precision motorized focusing

• Ni-E

High-precision Z-focus provides accurate Z-position information required for use with confocal laser microscopes and supports automated Z-series acquisition. Individual coarse and fine focus knobs enhance ease of operation.

CFI Plan Apochromat Lambda D series

• Ni-E
• Ni-L

The high-refractive index glass used in the oil-immersion objectives provides uniform brightness and high image quality up to the periphery of a large 25mm diagonal field of view, enabling efficient acquisition of seamless stitched images and supports macro imaging of large samples. High transmittance and chromatic aberration correction over a wide wavelength range from 405 nm to 850 nm enable reliable quantitative data acquisition for intensity analysis of nuclear staining. These high NA lenses are ideal for brightfield and DIC observations, as well as fluorescence and confocal observations.

Learn more about CFI Plan Apochromat Lambda D series

Water dipping objective lenses

• Ni-E
• Ni-L

CFI Apochromat NIR 40X W/60X W objectives with long working distances and high NA provide high transmission over the near-IR wavelength range. Axial chromatic aberration is corrected up to the near-IR range, enabling high-resolution images of minute structures of thick samples during IR-DIC observations.

CFI75 Apochromat 25XC W and CFI Plan Achromat100XC W objectives featuring high NA (1.10) and long working distance (2.00 mm at 25XCW, 2.50 mm at 100XCW) are corrected for chromatic aberration in the IR range. These objectives can capture crisp images of deep regions of thick samples by adopting a mechanism to compensate for changes in spherical aberration that occur at different temperatures and observation point depths.

Uniformly bright illumination

• Ni-E
• Ni-L

A built-in “fly-eye” lens ensures uniform and bright illumination of the field of view, from edge-to-edge, at any magnification.

Fluorescence noise elimination

• Ni-E
• Ni-L

Nikon's proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio is dramatically improved by eliminating stray light in the filter cubes, allowing images with weak fluorescent signals to be captured with high contrast and brightness.

High color rendering LED light source

• Ni-E
• Ni-L

The high color rendering LED light source built-into the Ni-L provides natural color reproducibility comparable to a halogen light source, as well as light uniformity, long life, and other advantages associated with LEDs, making it effective for observation of pathological specimens.