Highest performance optics for confocal imaging
A selection of high numerical aperture (NA) objectives are available, which provide chromatic aberration correction for UV to near-infrared.
Image courtesy of Laurence Pelletier Lab, LTRI
Objectives
CFI SR HP Plan Apochromat Lambda S 100XC Sil
By using silicone oil that has a refractive index closely matching that of live cells as its immersion liquid, this lens allows high resolution imaging of thick samples and is suitable for long-term time-lapse imaging.
- Numerical aperture: 1.35
- Chromatic aberration correction: from visible to UV
- Nano Crystal Coat applied.
CFI Plan Apochromat 10xC Glyc
This lens has a long working-distance providing ultra-deep imaging with maximum clarity and contrast. The support of multiple refractive indices allows observation with various optical clearing liquids as well as water, oil, and glycerin.
- Working distance: 5.5 mm
- Numerical aperture: 0.5
- Chromatic aberration correction: from visible to infrared.
- Nano Crystal Coat applied.
CFI Apochromat LWD Lambda S 20XC WI
This highly versatile objective provides superior performance thanks to its high numerical aperture, wide field of view and long working distance.
- Working distance: 0.95 mm
- Numerical aperture: 0.95
- Chromatic aberration correction: from visible to infrared.
- Nano Crystal Coat applied.
CFI Apochromat Lambda S 40XC WI
Its high NA for water immersion objectives provides brighter and higher-resolution images and makes this lens ideal for confocal live cell imaging.
- Numerical aperture: 1.25
- Chromatic aberration correction: from UV through to near IR
- Nano Crystal Coat applied.
CFI Plan Apochromat IR 60XC WI
This lens has the industry’s highest NA for a 60X water immersion objective lens, maintaining flatness throughout the field of view while showing good correction of chromatic aberration over a wide range of wavelengths.
- Numerical aperture: 1.27
- Super image flatness
- Chromatic aberration correction: from visible to infrared.
- Nano Crystal Coat applied.
CFI Apochromat TIRF 60XC Oil
This lens has the industry’s highest NA, providing unparalleled resolution and efficient acquisition of fluorescent signals in confocal imaging.
- Numerical aperture: 1.49
Nano Crystal Coat for superior transmission
Nikon’s exclusive Nano Crystal Coat is an anti-reflective coating consisting of ultra-fine crystalline particles. This forms a coarse structure that lowers the reflectance index, facilitating the passage of light through the lens rather than reflecting it, and thus providing superior light transmission.
Objectives for confocal microscopy
| CFI Plan Apochromat 10XC Glyc | NA 0.50 | WD 5.50 | Nano Crystal Coat |
|---|---|---|---|
| CFI Plan Apochromat Lambda 10X | NA 0.45 | WD 4.00 | Nano Crystal Coat |
| CFI Plan Apochromat Lambda 20X | NA 0.75 | WD 1.00 | Nano Crystal Coat |
| CFI Plan Apochromat Lambda 40XC | NA 0.95 | WD 0.21 | Nano Crystal Coat |
| CFI Plan Apochromat Lambda 60XC | NA 0.95 | WD 0.21-0.09 | Nano Crystal Coat |
| CFI Plan Apochromat Lambda 60X Oil | NA 1.40 | WD 0.13 | Nano Crystal Coat |
| CFI Plan Apochromat Lambda 100X Oil | NA 1.45 | WD 0.13 | Nano Crystal Coat |
| CFI Plan Apochromat VC 20X | NA 0.75 | WD 1.00 | |
| CFI Plan Apochromat VC 60XC WI | NA 1.20 | WD 0.31-0.28 | |
| CFI Plan Apochromat VC 100X Oil | NA 1.40 | WD 0.13 | |
| CFI Plan Apochromat IR 60XC WI | NA 1.27 | WD 0.18-0.16 | Nano Crystal Coat |
| CFI SR Plan Apochromat IR 60XC WI | NA 1.27 | WD 0.18-0.16 | Nano Crystal |
| CFI SR HP Plan Apochromat Lambda S 100XC Sil | NA 1.35 | WD 0.30 | Nano Crystal Coat |
| CFI Apochromat Lambda S 40XC WI | NA 1.25 | WD 0.20-0.16 | Nano Crystal Coat |
| CFI Apochromat Lambda S 60X Oil | NA 1.40 | WD 0.14 | Nano Crystal Coat |
| CFI Apochromat LWD Lambda S 20XC WI | NA 0.95 | WD 0.95 | Nano Crystal Coat |
| CFI Apochromat LWD Lambda S 40XC WI | NA 1.15 | WD 0.62-0.58 | Nano Crystal Coat |
| CFI Apochromat TIRF 60XC Oil | NA 1.49 | WD 0.14-0.08 (23℃) WD 0.16-0.10 (37℃) | |
| CFI Apochromat TIRF 100XC Oil | NA 1.49 | WD 0.14-0.08 (23℃) WD 0.15-0.09 (37℃) | |
| CFI75 Apochromat 25XC W | NA 1.10 | WD 2.00 | Nano Crystal Coat |
Confocal and Multiphoton Microscopes
- AX / AX R with NSPARC
- AX R MP with NSPARC
- A1 HD25 / A1R HD25
- Yokogawa CSU Series
