Perfect for imaging 3D cell cultures and thick tissue samples. Silicone lenses enable visualization of cellular dynamics clearly and brightly when imaging at depth.

Research applications in neuroscience and cell biology using brain tissue, spheroids, organoids, and 3D cultures continue to push the limits of imaging in thick specimens. The need to image deep into such samples has never been more apparent. Nikon’s silicone immersion objectives enable clear observation with high signal-to-noise deep into living tissue.

The silicone immersion lens series features wide fields of view, high resolution, and evaporation-resistant oil, facilitating observations with ease. 

With the addition of the new 60X objective, which employs newly developed glass for enhanced chromatic aberration correction, quantitative imaging in thick living specimens has never been more attainable.

Folheto

Baixar Silicone Immersion Objective Series Brochure (3.13MB)

Características principais

Efficiently switch magnification for macro-to-micro imaging without changing the immersion medium

SIL25X

SIL25X/1.05
Zoom 1X, Pixel 0.69 μm

SIL25X/1.05
Zoom 5X, Pixel 0.14 μm

SIL40X

SIL40X/1.25
Zoom 1X, Pixel 0.43 μm

SIL40X/1.25
Zoom 4X, Pixel 0.11 μm

SIL60X

SIL60X/1.30
Zoom 3X, Pixel 0.10μm

SIL60X/1.30
Zoom 1X, Pixel 0.29 μm

Small intestine enteroid (Cadherin: Alexa Fluor® 555, Nuclear: DAPI)
Images courtesy of: Dr. Yuki Yokoi, Dr. Kiminori Nakamura, and Dr. Tokiyoshi Ayabe
Innate Immunity Laboratory, Department of Cell Biological Science, Faculty of Advanced Life Science
Graduate School of Life Science, Hokkaido University

SIL100X

SIL100X/1.35
Zoom 1.6X, Pixel 0.11 μm

SIL100X/1.35
Zoom 1X, Pixel 0.17 μm

Make high-resolution deep observations of thick specimens such as 3D cell cultures, organoids, and tissues

2D image surface

Deep 2D image

Small intestine enteroid (Cadherin: Alexa Fluor® 555, Nuclear: DAPI)
Images courtesy of: Dr. Yuki Yokoi, Dr. Kiminori Nakamura, and Dr. Tokiyoshi Ayabe
Innate Immunity Laboratory, Department of Cell Biological Science, Faculty of Advanced Life Science
Graduate School of Life Science, Hokkaido University Science, Hokkaido University

Observe live samples over long periods of time

It is also best suited for long time-lapse imaging of live cells without the immersion medium evaporating because silicone oil has low volatility even at 37°C.

Time-lapse imaging of enteroid (25X)
Images courtesy of: Dr. Yuki Yokoi, Dr. Kiminori Nakamura, and Dr. Tokiyoshi Ayabe
Innate Immunity Laboratory, Department of Cell Biological Science, Faculty of Advanced Life Science
Graduate School of Life Science, Hokkaido University

By using the Denoise.ai in conjunction with the AX confocal microscope, you can acquire even sharper images.

Before Denoise.ai processing

After Denoise.ai processing

Newly developed Short-wavelength Refractive (SR) glass

The new silicone immersion 60X objective employs high- and specialized-dispersion glass that was independently developed by Nikon and possesses extra-low dispersion properties.

By refracting short-wavelength light to a higher degree, it is possible to collect a wider range of wavelengths, resulting in significantly enhanced chromatic aberration correction. In addition to axial chromatic aberrations, lateral chromatic aberrations can also be corrected.

Using only normal lens

With SR Lens

*The photo is a sample image

Useful for a variety of scientific disciplines such as immunology, developmental biology, neuroscience, drug discovery and regenerative medicine.

Mouse nerves
Images courtesy of: Lin Daniel, PhD. SunJin Lab Co.

Mouse synapse

Neutrophil flowing in blood vessel (time-lapse)
Images courtesy of: Professor Masaru Ishii, Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University

Spheroid

Small intestine organoid
Image courtesy of: Dr. Hidenori Akutsu and Dr. Tomoyuki Kawasaki of the Center for Regenerative Medicine, National Center for Child Health and Development

Fertilized mouse embryo
Image courtesy of: Dr. Yoshiteru Kai, Reproductive Medicine Research Center, YAMASHITA SHONAN YUME CLINIC

Dendrite