Notes d'application

Confocal imaging of vasculature network using the HUMIMIC Chip

mars 2023

In this application note, we introduce 3D imaging of a vascular network formed in TissUse’s HUMIMIC Chips. By visualizing actin filaments (F-actin) and a CD31 marker of endothelial cells with fluorescence, and observing them with a confocal microscope, we confirmed that 3D tubular network structures were formed in the cell culture compartments. The vascularization will enable the physiological culture conditions for spheroids or organoids in the chips (e.g capillarization of spheroids/organoids).