Notes d’application

A Non-Invasive Method for Counting Human Pluripotent Stem Cell Numbers by Live Cell Imaging

janvier 2012

Presented here is a method for counting live pluripotent stem cells using the fluorescent nuclear stain SYTO24 and phase contrast imaging on the Nikon BioStation CT high content screening microscope. The results show that colony coverage area deduced from phase contrast images can be used as an accurate estimator of stem cell count alone, correlating well with haemocytometer-based measurements.