Confocal and Multiphoton Microscopes

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Confocal microscopy provides optical sectioning, the ability to observe discrete planes in 3D samples, by using one or more apertures to block out-of-focus light. Nikon offers both point-scanning confocal instruments, led by our AX / AX R confocal system, as well as spinning disk (field scanning) confocal systems from top manufacturers.

Multiphoton microscopy is preferred for deep imaging applications in thick specimens, including intravital imaging. Non-linear excitation restricts fluorescence to the laser focus and near-infrared illumination minimizes absorption and scattering. Nikon offers the AX R MP multiphoton system, available with microscope stand options optimized for large specimens.

Image scanning microscopy (ISM) is a super-resolution technique that takes advantage of structured detection of each point in a point-scanning system to improve both resolution and signal-to-noise (S/N), a great choice for low light imaging. Both the AX / AX R confocal and AX R MP multiphoton systems may be equipped with the Nikon Spatial Array Confocal (NSPARC) detector for ISM imaging.

AX Series

AX/AX R

Widefield, super-resolution confocal laser scanning microscope

— the core of the AX series —

AX R MP with NSPARC

Achieves super-resolution and deep imaging while realizing low-noise images in deep observation via the NSPARC detector.

  • Widefield
  • High speed
  • High resolution
  • Deep imaging
  • Long wavelength
  • Analysis
  • Minimally invasive
  • Low phototoxicity
  • In Vivo

AX R MP

Provides a wide field of view and fast imaging that are ideal for clear visualization of deep microstructures.

  • Widefield
  • Long wavelength
  • High speed
  • Deep imaging
  • Analysis
  • Minimally invasive
  • Low phototoxicity
  • In Vivo

AX / AX R with NSPARC

Equipped with an NSPARC detector for super-resolution, achieving exceptionally low-noise and high-spatial-resolution imaging.

  • Widefield
  • High speed
  • High resolution
  • Analysis
  • Minimally invasive
  • Low phototoxicity
  • Multicolor

AX NIR

Realizes high wavelength selectivity with near-infrared excitation, making it ideal for live-cell long-wavelength imaging.

  • Widefield
  • High speed
  • Analysis
  • Minimally invasive
  • Low phototoxicity
  • Multicolor+NIR
  • In Vivo

Product Lineup

AX / AX R with NSPARC

Nikon’s latest confocal microscope, leveraging deep learning-based AI tools to simplify acquisition and improve image quality. Fast scanning combined with a large 25 mm field of view enables high throughput imaging with unmatched edge-to-edge image quality. Super-resolution provided by the NSPARC detector.

  • Research
  • Biotech

AX R MP with NSPARC

The newest multiphoton microscope from Nikon is a top choice for deep imaging. The next generation 2K resonant scanner captures fast in vivo dynamics with high resolution over a large field of view. The AX R MP also supports confocal imaging with visible wavelengths and super-resolution using the NSPARC detector.

  • Research

A1 HD25 / A1R HD25

Confocal microscope featuring the world’s largest 25 mm field of view for high-throughput, high-resolution imaging.

  • Research
  • Biotech

Yokogawa CSU Series

Spinning disk confocal systems from Yokogawa Life Sciences provide fast and user-friendly options for live cell, organoid, and other imaging applications. The CSU-W1 SoRa spinning disk system provides super-resolution using Optical Pixel Reassignment (OPR) technology.

  • Research
  • Biotech

Application Notes

Product Brochures

AX / AX R with NSPARC Brochure

Download 15.68MB

AX R MP with NSPARC Brochure

Download 10.84MB