ECLIPSE Ji | Digital Microscopes | Microscope Products | Nikon Corporation Healthcare Business Unit

Research Microscope Power in a Benchtop Assay Instrument

Simple Operation

Minimal experimental setup and complexity by AI-driven assays and analysis.

Easy Cellular Imaging

No need to master complicated microscope hardware and software – ECLIPSE Ji makes data collection and cellular imaging assays easy.

High Quality Optics

Renowned Nikon optics provide clear and sharp images on plate assay devices.

Brochures

Download ECLIPSE Ji (for Assays) (4MB)

Download ECLIPSE Ji (for Research) (3.63MB)

Key Features

Smart Experiments with Automated Assays

Utilizing Nikon’s precision optical hardware, all of the advantages of high sensitivity and resolution from a research-level microscope are retained by an AI-driven, easy-to-operate benchtop laboratory instrument.

Preconfigured and optimized turnkey assay experiments minimize time spent defining parameters, maximizing data collection.

Standard Assays

Intensity Measurement

Compares protein expression level changes in cells and cell nuclei in multiple wells.

Application Note

Cell Counting (endpoint)

Counts the number of cell nuclei in a fixed sample and the area of the well occupied by cells.

Application Note

Cell Counting (proliferation)

Measure changes in confluency and count nuclei in live samples over time.

Transfection Efficiency

Investigates the percentage of cells expressing the target protein, and reports the expression efficiency.

Application Note

Size & Morphological analysis

Analyzes cellular morphology with measurements of the cell nucleus, cytoplasm, and the size of the cell region.

Application Note

Cytotoxicity

Measures the percentage of dead cells among all cells and evaluates cytotoxicity.

Application Note

Optional Assays

Apoptosis

Measures the percentage of apoptotic cells among the population.

Application Note

DNA damage (gamma H2AX)

Analyzes DNA damage foci within individual cell nuclei.

Application Note

Nuclear Translocation

Measures the nuclear translocation of NF-κB, indicating an extra-cellular stimulus.

Application Note

Autophagy

Measures the number of autophagosomes, their area, and their fluorescence intensity.

Application Note

Phagocytosis

Measures the number, area, and fluorescence intensity of bioparticles taken into the cell by phagocytosis.

Application Note

Endocytosis

Measures the number, area, and fluorescence intensity of granules formed by endocytosis, which are taken up from outside the cell.

Application Note

Micronucleus test

Measures the number of cells containing micronuclei or multiple nuclei.

Application Note

Mitochondrial toxicity

Measures the number, area, and fluorescence intensity of mitochondria.

Application Note

Neurite Outgrowth

Measure the number and length of neurites protruding from neuron cell bodies.

Wound Healing

Measure the recovery of filled area in an artificially-created wound over time.

Cell Cycle (Fucci)

Measure the proportion of cells in each cell cycle phase based on Fucci, the genetically-encoded, fluorescent cell cycle reporter system.

Effortless Results using AI

ECLIPSE Ji’s Smart Experiment software interface uses newly developed artificial intelligence (AI), implemented to minimize errors and maximize data collection.

Input	Output
Load a plate	Select assay / Input basic information	Check the shooting results and analysis results	Report
step 1	step 2	step 3	step 4
ECLIPSE Ji	Pre-Acquisition AI automatically judges plate status and automatically adjusts acquisition conditions	Actual Acquisition/analysis/data display Automatic acquisition, data extraction, graphing under optimized acquisition conditions	Reporting One click export of the data

Input

Output

Load a plate

Select assay / Input basic information

Check the shooting results and analysis results

Report

step 1

step 2

step 3

step 4

ECLIPSE Ji

Pre-Acquisition

AI automatically judges plate status and automatically adjusts acquisition conditions

Actual Acquisition/analysis/data display

Automatic acquisition, data extraction, graphing under optimized acquisition conditions

Reporting

One click export of the data

Single-Cell Imaging and Analysis with Ease

AI based on Deep Learning defines acquisition settings and image analysis parameters, saving researchers valuable time at the microscope.

Automatic Plate Detection

Plate type and dimensions are automatically detected. There is no need to select from lists or manually enter plate data.

Automatic detection of samples

A rapid preview across the entire plate determines which wells have sample present, allowing users to easily skip empty wells.

Automatic calculation of optimal exposure settings

There is no need for troublesome tuning of light intensity and exposure time. The optimal exposure settings for image analysis are automatically calculated from the luminance values of all wells.

Automatic alignment of the plate

No alignment work is required. The system automatically detects and corrects the plate position.

User Interface Designed for Data-Rich Microscopy

Images and corresponding analysis data for the plate, well, and each cell is contained in an interactive and linked interface. Users can navigate and quickly visualize trends and results.

ECLIPSE Ji: a Multi-Role Digital Inverted Microscope

Outside of plate assays, ECLIPSE Ji can also serve as a digital research microscope, and can be integrated with a variety of peripheral hardware ranging from filter wheels through confocal systems such as "AX", or high-sensitivity cameras.