Application Notes

Analysis of nuclear translocation using AI-driven fully automated ECLIPSE Ji

September 2023

ECLIPSE Ji with Smart Experiment software enables seamless experiments from image acquisition to analysis and graph creation. Pre-trained Artificial Intelligence (AI) and pre-defined imaging processes automatically optimize image acquisition and analysis settings, providing visualized data and EC50 information with simple operation. The transcription factor NF-κB is widely known as a drug target for cancer and inflammatory diseases. Activated NF-κB translocates from the cytoplasm to the nucleus and induces the expression of disease-related genes. This application note introduces an example of using the Nuclear Translocation module of Smart Experiment to quantify the TNF-α-stimulated NF-κB translocation from the cytoplasm to the nucleus.