Application Notes

Calculating the percentage of cells with micronuclei using the AI-driven, fully automated smart imaging system ECLIPSE Ji

Januar 2024

Genotoxic substances are known to induce chromosomal aberrations which cause micronuclei to form during cell division. Detection of these micronuclei via microscopy is useful for characterizing the genotoxicity of drug candidates and other compounds. The ECLIPSE Ji digital inverted microscope equipped with NIS-Elements Smart Experiment (SE) software enables automated, seamless imaging workflows from acquisition to analysis and data visualization. This application note introduces the Micronucleus Test SE module, which was used to determine the percentage of cells containing micronuclei in response to the genotoxic compound Mitomycin C.