Application Notes
A practical assessment of analytical performances for pseudoislet morphology by GA3 and other image analysis tools
März 2025
The advent of three-dimensional (3D) cell cultures, including spheroids and organoids, has revolutionized cell biology research by providing more accurate tissue representations. However, the cultivation and analysis of these complex structures present significant challenges. In their recent research publication, Dr. Rademakers and colleagues evaluated three microwell culture plate designs and four image analysis software packages, including Nikon's GA3, for their efficacy in 3D culture analysis. Their findings reveal that Nikon's GA3 demonstrated superior versatility and accuracy in quantifying nuclei and cells within 3D cultures. It also excelled in classifying cell localization, outperforming open-access alternatives that often require extensive post-processing optimization.
This application note provides critical insights for researchers employing microscopic imaging in 3D cell culture analysis, highlighting the advantages of Nikon's advanced imaging systems and software solutions. Their findings underscore the importance of selecting appropriate tools and methodologies to maximize the potential of 3D cell culture research in advancing biological understanding.