Application Notes

Analysis of phagocytosis using the AI-driven, fully automated smart imaging system ECLIPSE Ji

Januar 2024

Phagocytosis is the process by which immune cells take in and degrade extracellular foreign substances, such as dead cells and bacteria. The ECLIPSE Ji digital inverted microscope equipped with NIS-Elements Smart Experiment (SE) software enables automated, seamless imaging workflows from acquisition to analysis and data visualization. This application note introduces the Phagocytosis SE module, which was used to quantify the phagocytic activity of J744.1 cells by measuring the fluorescence intensity and localization of fluorescently labeled E. coli (K-12 strain).