Application Notes

Automated, Non-Invasive Culture, and Evaluation System for iPS Cells under Neural Differentiation Process

January 2011

This application note details an integrated workflow for the culture, observation, evaluation, and differentiation of induced pluripotent stem (iPS) cells into neurons, introducing image processing methods for detecting neural rosettes and neurite outgrowth – two important morphological checkpoints in neural differentiation. Automated imaging for this assay is performed using a Nikon BioStation CT.