Application Notes
피질 흥분성 생쥐 뉴런의 3D 해상도 향상 공초점 이미징으로 역학, 조절 및 기능이 각기 다른 가지돌기 작은 가시의 부분 집합 확인
2021 년 11 월
In this application note, we used strong fluorescence labeling of neuronal elements and optimized Nikon laser scanning confocal imaging parameters, followed by post-acquisition iterative 3D deconvolution using NIS-Elements software (Zaccard, et al., 2021). This technique enabled the visualization and tracking of individual spinules in relation to presynaptic and postsynaptic markers, revealing spinule subtypes that differ in dynamics, length, lifespan, regulation, and function (Zaccard et al., 2020).