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UT Austin

The Center for Biomedical Research Support Microscopy and Imaging Center is dedicated to supporting the research community at UT Austin and beyond by providing access to state-of-the-art imaging instrumentation, as well as technical assistance and education, to researchers across all disciplines.

Opening: Wednesday, March 6, 2019; From left to right: Andy Davis (General Manager, Sales, Nikon Instruments Inc.), Toshiaki Nagano (President & Chief Executive Officer, Nikon Instruments Inc.), Daniel Jaffe (Vice President for Research, The University of Texas at Austin), Andreas Matouschek (Professor, Core Facilities Director, Molecular Biosciences, Office of the VP for Research, The University of Texas at Austin)

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CofE Director

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Address

The University of Texas at Austin
MBB 1.426
2500 Speedway A4800 Avenue
Austin TX 78712

Website

UT Austin



Systems Available

A1R MP Resonant Scanning Multiphoton System

This open architecture system based on the Ni upright microscope features the A1R MP resonant scanning multiphoton system as well as visible light detection for a variety of challenging samples.

Components

  • Open architecture system based on the Ni upright microscope for accomodating large equipment and samples
  • A1R MP resonant scanning multiphoton system
  • LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
  • Coherent Chameleon Discovery dual beam laser unit (680-1300nm)
  • Episcopic GaAsP non-descanned detectors (NDDs)
  • DUV-B 2-channel spectral GaAsP detector
  • NIS-Elements software

A1R Resonant Scanning Confocal System

This A1R resonant scanning confocal system is configured on a Ti2-E inverted microscope and features stage-top incubation for live cell imaging.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • A1R resonant scanning confocal system
  • LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
  • DU4G GaAsP 4-channel confocal detector
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

N-STORM/TIRF Super-Resolution System

The N-STORM provides super-resolution single molecule localization imaging on the Ti-E inverted microscope, and also features emission splitting optics for fast live-cell and multicolor TIRF imaging.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • N-STORM super-resolution single molecule localization microscopy system
  • LUN-V high power 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Cairn Research TwinCam two-camera emission splitter for fast two-color TIRF imaging
  • Andor iXon 897 EMCCD camera
  • Hamamatsu ORCA-Flash4.0 V3 sCMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

CSU-W1 Spinning Disk Confocal System

The CSU-W1 provides fast spinning disk confocal performance with large fields of view and deep in samples. Emission splitting optics and incubation unlocks a number of live cell imaging applications.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • Yokogawa CSU-W1 spinning disk confocal
  • Toptica Photonics iChrome MLE 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • 2x Andor iXon 888 EMCCD camera
  • Large format DS-Qi2 monochrome CMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

Large FOV High Content Screening System

This large field of view and high content screening system is configured on a Ti2-E inverted microscope.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • Photometrics Prime95B sCMOS camera
  • Large format DS-Ri2 color CMOS camera
  • Lumencor SPECTRA X light engine
  • Lumencor PEKA light engine
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Okolab stage-top incubation system
  • NIS-Elements software

Upright Widefield Microscope System

This Ni-E upright microscope system features both monochome sCMOS and color CCD cameras, supporting a variety of observation modes.

Components

  • Ni-E upright microscope
  • Andor Zyla 4.2 sCMOS camera
  • DS-Fi3 color CCD camera
  • Lumencor SOLA light engine
  • NIS-Elements software

Stereomicroscope System

The SMZ25 stereomicroscope features world-leading 25:1 zoom ratio and this system additionally features a color CMOS camera and NIS-Elements software for recording observations.

Components

  • SMZ25 motorized stereomicroscope
  • P-DSL32 LED diascopic stand with oblique coherent contrast (OCC)
  • Large format DS-Ri2 color CMOS camera
  • Lumencor SOLA light engine
  • NIS-Elements software

Inverted Widefield Microscope System

This Ts2R-FL is a workhorse inverted microscope for a variety of routine fluorescence imaging applications.

Components

  • Ts2R-FL inverted fluorescence microscope
  • Lumencor SPECTRA X light engine
  • Photometrics CoolSNAP DYNO CCD Camera
  • NIS-Elements software