®®Intracytoplasmic morphologically selected sperm injection (IMSI) is a technique in the in vitro fertilization (IVF) field used to select the healthiest sperm for intracytoplasmic sperm injection (ICSI). This level of morphological scrutiny requires very high magnification and sharp contrast. Nikon’s CFI Plan LWD IMSI 100X dry objective features a correction collar, DIC compatibility, and can be used with the same polarizer as Nikon’s NAMC optics, which makes switching between multiple objectives and contrast techniques simple.
| Model | Dimensions | Transmittance | NA | W.D. (mm) | Cover glass thickness | Correction ring | Observation |
|---|---|---|---|---|---|---|---|
| CFI Plan Achromat LWD IMSI 100XC | Diagram | Graph | 0.85 | 1.30 -0.95 (0.90 with cover glass thickness of 0.9 mm) | 0.60 - 1.30 | ∨ | BF, DF (Oil), DIC, POL, FL (visible light) |
BF: Brightfield
DF: Darkfield
DIC: Differential Interference Contrast
POL: Simple Polarizing
FL: Fluorescence
