Centro de excelencia

University of Minnesota

The University Imaging Centers (UIC) serve internal and external research clients in the design of imaging experiments, choice of and training on suitable imaging systems, and subsequent image processing, visualization and analysis. The Centers provide a number of advanced imaging systems including: N-SIM super-resolution microscopy; macro spectral confocal microscopy; wide-field light and fluorescence microscopy; laser-scanning confocal microscopes; total internal reflectance microscope (TIRF); laser capture micro-dissection; several live cell imaging systems; poster printers; full sample preparation capabilities; along with 4 full-time and 4 part-time experienced staff members.

The UIC operates on the following fundamental mission goals:

Maximize the availability of advanced imaging technologies and methodologies to faculty, staff, students and external users.
Bring expertise in new imaging technologies to users.
Educate the University community about new imaging technologies.
Promote interaction between developers of new imaging technology and potential users.

Contacto

CofE Director

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Address

University of Minnesota
1-151 Jackson Hall, 321 Church Street SE
Minneapolis, MN 55455
(612) 624-7938

Website

University of Minnesota

Systems Available

A1si Point Scanning Confocal System

This A1si point scanning confocal system is configured on a Ti2-E inverted microscope and features a spectral detector for linear unmixing of signals from multiple emitters.

Components

Sistema confocal y de súper resolución de disco giratorio CSU-W1 SoRa
A1si point scanning confocal system
A1-DUS 32-channel spectral detector
DU4 4-channel confocal detector
LU4A 7-line laser unit (405nm, 457nm, 477nm, 488nm, 514nm, 561nm, 638nm)
Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
NIS-Elements software

A1R Resonant Scanning Confocal with FLIM Module

This system features an A1R resonant scanning confocal system with Picoquant FLIM module for fluorescence lifetime imaging applications.

Components

Ti-E inverted microscope with Perfect Focus System (PFS)
Picoquant FLIM module
LUN-V 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
3-line laser unit (405nm, 485nm, 560nm) for FLIM
Hamamatsu ORCA-Flash4.0 V3 sCMOS camera
DU4G GaAsP 4-channel confocal detector
NIS-Elements software

A1R HD MP Resonant Scanning Multiphoton System

This A1R HD MP based resonant scanning system provides both fast multiphoton and visible imaging on one microscope.

Components

FN1 upright microscope
A1R HD MP multiphoton system with high-definition resonant scanner
Spectra Physics Mai Tai eHP DeepSee Ti:S laser (tunable 690 - 1040nm) for multiphoton
6-line laser unit (405nm, 457nm, 488nm, 514nm, 561nm, 637nm) for confocal
Episcopic non-descanned detectors (NDDs)
NIS-Elements software

N-SIM/A1Rsi Super-Resolution & Confocal System

Fast confocal and super-resolution on the same Ti-E inverted microscope with the A1Rsi resonant scanning confocal and N-SIM structured illumination systems.

Components

Ti-E inverted microscope with Perfect Focus System (PFS)
A1Rsi resonant scanning confocal system
N-SIM super-resolution structured illumination microscopy system
LUN-V 4-line laser unit (405nm, 488nm, 561nm, 640nm) for confocal
LU5 N-SIM 4-line laser unit (405nm, 488nm, 561nm, 647nm) for SIM
A1-DUS 32-channel spectral detector
DU4G GaAsP 4-channel confocal detector
Andor iXon 897 EMCCD camera
NIS-Elements software

AZ-C1si Macro Confocal System

This unique macro confocal system combines the AZ100 multizoom microscope with the C1si point-scanning confocal system with spectral detector.

Components

AZ100 multizoom microscope
C1si point scanning confocal system
LU4A 7-line laser unit (405nm, 458nm, 476nm, 488nm, 515nm, 561nm, 638nm)
C1-SHS 32-channel spectral detector
DU3 3-channel confocal detector
NIS-Elements software

AZ100M Motorized Multizoom Microscope

The AZ100M is the motorized version of the multizoom microscope/macroscope, and features multiple cameras and NIS-Elements software for recording observations.

Components

AZ100M motorized multizoom microscope
Photometrics CoolSNAP ES2 CCD camera
DS-Ri1 color CCD camera
NIS-Elements software

BioStation IM-Q System

The BioStation IM-Q incorporates a microscope, an incubator and a high-sensitivity cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition.