Capture low light fluorescence and large fields of view.
Equipped with a Nikon digital SLR camera FX-format CMOS sensor optimized for microscopy, the DS-Qi2 is an ultra-high quality 16.25 megapixel monochrome camera that features high pixel density, high sensitivity and low noise. The DS-Qi2 is an excellent choice for applications in quantitative fluorescence imaging.
* DS-Qi2 is not for clinical diagnostic use. This product may no longer be available in some regions. Please contact your local Nikon dealer for details.
Key Features
Large format CMOS sensors
Nikon manufactures CMOS sensors and imaging technologies for professional D-SLR cameras, which are now optimized for microscopy.
High sensitivity
Detects even faint fluorescent signals
7.3µm pixels, high quantum efficiency, and very low read noise allow the DS-Qi2 to even read faint fluorescent signals.
Pig kidney epithelial cells expressing GFP-EB3 tubulin
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University
Excellent linearity
Reliable quantitative analysis made possible
With a linearity error of ±1%, the DS-Qi2 is a superb tool for measuring intensities in fluorescence samples, including time-based intensity measurement and ratiometric measurement.
Low noise
Acquires dim fluorescent signals with ultra-low noise
2.2 electrons read noise, coupled with a large full-well capacity, allows for the acquisition of fluorescence images with very little noise and a very high dynamic range.
LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.
A captured image is shown intensity scaled to show both the brightest and dimmest areas.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University
Time-lapse photography
Fluorescent time-lapse imaging through integration with NIS-Elements software
With a large field of view, high pixel density, and low noise, the DS-Qi2 is ideal for time-resolved imaging applications.
Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.
DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.
Objective: CFI Plan Apochromat Lambda 60X Oil (NA: 1.4)
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University
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