Driving cells with light-controlled topographies
14:30 EDT / 19:30 BST / 20:30 CEST
Alberto Puliafito, Ph.D
Department of Oncology, University of Torino, 10043 Orbassano, Torino, Italy. Candiolo Cancer Institute FPO-IRCCS, 10060 Candiolo, Torino, Italy.
Prof. Emiliano Descrovi, Ph.D.
Department of Applied Science and Technology, Politecnico di Torino, 10129 Torino. Department of Electronic Systems, Norwegian University of Science and Technology – NTNU, N-7491 Trondheim, Norway
14:30 - 15:30 CEST
In this webinar we will show how to mechanically stimulate cells adhering on a substrate. We will focus on the use of a widefield microscope to both deform the substrate where cells are growing and to monitor cell changes over time. Particular attention will be devoted to quantitative image analysis needed to characterize cell response upon mechanical stimuli. An overview of image analysis techniques such as segmentation and tracking will be presented.