14.ObservationMake observations using transmitted light fluorescence microscopy
Make observations using transmitted light fluorescence microscopy
What is transmitted light fluorescence microscopy?
Transmitted light fluorescence microscopy is a microscopic method that uses transmitted light when observing specimens labeled with a fluorescent dye or fluorescent protein.
By lighting transmitted light in specific wavelengths to the objects dyed with the special fluorescent dye, the excited fluorescence can be observed as an image.
Preparation to use transmitted light fluorescence microscopy
Make sure the following components are usable.
EX filter slider
When using light field microscopy, place the BF position in the optical path. When using transmitted light fluorescence microscopy, place the GFP position in the optical path.
BA filter slider
When using light field microscopy, pull it to the click position and put the empty hole into the optical path. When using transmitted light fluorescence microscopy, push it to the limit, and put the BA filter in the optical path.
Objective lens for transmitted light fluorescence microscopy
It is possible to use CFI E Plan 4x, CFI E plan 10x, CFI E Plan 40x objective lenses for transmitted light fluorescence microscopy.
Make observations using transmitted light fluorescence microscopy
Remove the dummy filter slider and put the BA filter slider in the position.
Remove the dummy slider and put the EX filter slider in the position.
Fully open the condenser aperture diaphragm lever, and adjust the stage height with the stage movement knob.
Adjust the height of the stage with the coarse focus knob. Then, observe after using the fine focus knob to adjust the focus.
