12.ObservationMake observations using light field microscopy
Make observations using light field microscopy
The observing point of light field microscopy
Focus on the specimen using a low magnifying power objective (4X or 10X) and observe the entire image.
Search by operating focus knob and stage movement handle.
Do not switch the objective lens directly from 4X to 60X or 100X. Before switching to the higher-magnification objective lenses, make sure to focus on with the objective lens of 10X or more.
To observe details
Move the point you want to observe in detail to the center of the viewfield. To look at the specimen in closer detail, increase the magnification of the objective (20X or 40X objectives), adjust the focus, and observe.
Observe after matching the aperture diaphragm lever to the same figure as the magnifying power of the objective.
To make observations using the maximum resolution
To make observations using the maximum resolution, use oil immersion objectives, and the maximum numerical aperture. Refer to item "Oil immersion" for oil immersion objectives and oil immersion observations.