13.ObservationMake observations using phase contrast microscopy
Make observations using phase contrast microscopy
What is phase contrast microscopy?
Phase contrast microscopy is a technique that uses transmitted illumination to observe colorless and transparent and unstained specimens such as living cells without staining.
Insert a phase contrast slider with a diaphragm ring in the slot on the condenser side. It becomes phase contrast microscopy by inserting a phase contrast objective lens with the same PH code into the optical path.
Preparing for phase contrast microscopy
Please ensure that the following optical components are usable.
10X-40X slider (Microscope slider for phase contrast)
This slider is for 10X, 20X and 40X phase contrast objective lenses. When switching to phase contrast microscopy, remove the dummy slide from the condenser slide slot, and insert this slide.
100X slider (Microscope slider for phase contrast)
This slider is for 100X phase contrast objective lens. Since there is an empty hole, enter the empty hole in the optical path beforehand. When switching to phase contrast microscopy, move the slider, and insert it into the position of the optical path.
Objective lens for phase contrast microscopy (Ph objective lens)
Make sure the objective lens is attached to the revolver When attaching the lens to the revolver, ensure that the slider, the PH code of the objective lens, and NA can be aligned correctly.
Table for the corresponding phase contrast sliders and objective lenses
Phase contrast slider
Objective lens
PH code
Corresponding NA
10x-40x
PH1
0.25〜0.5
PH2
0.55〜8.5
100x
PH3
0.9〜1.4
Centering telescope
Used to adjust the positioning of the diaphragm ring.
Make observations using phase contrast microscopy
Raise the stage, adjust the condenser vertical motion lever, and remove the condenser.
Remove the dummy slider from the condenser that was taken out, and insert the slider for phase contrast microscopy.
Return the condenser and open the condenser aperture diaphragm lever fully.
Make sure the PH1 letters can be seen, then push the slider until you hear a click.
Adjust the field diaphragm dial the condenser vertical movement knob to focus.
Attach the centering telescope and adjust the field of vision, using your right eye to center it.
Replace it with a 40X objective lens, and while looking into the centering telescope, use the fine adjustment knob to bring it back into focus.
Change the slider to the PH4 side, and bring it back into focus.
Re-adjust the condenser lens and use your right eye to center it.
Return the objective lens to 10X, remove the centering telescope, and embed the eyepiece.
Bring it into focus, open the view aperture dial, and observe.
Replace it with a 40X objective lens and focus it.
Adjust the focus and narrow the aperture to observe.
