AX R MP with NSPARC

Système de microscope multiphotonique

Caractéristiques

Main body

AX-FNSP

AX-FNGP

Optical system

Infinity optical system

Microscope stands

AX-FNSP Single Stand

AX-FNGP Gate Stand

Focusing

AX-FN Focusing Nosepiece Unit
Motorized coaxial coarse/fine focusing
Focusing stroke: Up 13 mm/Down 2 mm*1, *2, Minimum step: 0.02 μm, Motorized escape and refocus mechanism
Focal plane: 400 mm above the surface of the vibration isolated table

Controls

AX-FNCTL Control Box
AX-FNHC Hub Controller (For controlling Focusing Nosepiece Unit, Diascopic Illumination System, Stage Joystick, Motorized Epi-fluorescence Cube Turret, Motorized Quadrocular Tilting Tube 2 and DSC Zooming Port)

Stages

AX-FNSP

AX-FNGP

Adapter

AX-FNSA Stage Adapter, supporting both manual and motorized XY stages. Stage height: adjustable to 2 positions depending on sample size (400 mm/200 mm from the surface of the vibration isolated table)

Stage

FN-3PS2 XY stage, Cross travel 29.5 (X) x 29.5 (Y) mm, with 2 auxiliary plates
AX-FNS-E Motorized XY stage, Cross travel ±34 (X) x ±27 (Y) mm

Epi-fluorescent illuminator

AX-FNSP

AX-FNGP

Illumination unit

NI-FLEI-2 Epi-fluorescence attachment

Light source

D-LEDI Fluorescent LED Illumination System

Filter cube turret

6 mountable filter cubes, shutter function

  • NI-FLT6 Epi-fluorescence Cube Turret
  • NI-FLT6-I Intelligent Epi-fluorescence Cube Turret
  • NI-FLT6-E Motorized Epi-fluorescence Cube Turret

Photostimulation device

AX-FNBPU Stimulation Back Port, 6 mountable filter cubes, Fluorescence imaging and simultaneous stimulation imaging can be switched

Diascopic illuminator

AX-FNSP

AX-FNGP

Illumination unit

AX-FNDIA Diascopic Unit
4 filter slider attachable, Condenser holder stroke: Up 2.5 mm/Down 1.8 mm, NI-PT Polarizer Turret mountable

Light source

C-LL High Color Rendering LED Lamphouse

Shutter

NI-SH-E Motorized Shutter

Condenser

FN-C LWD condenser, O.D. 8.2 mm, NA: 0.78

Polarizer Turret

NI-PT Polarizer Turret, Visible or infrared polarizer attachable

 

AX-FNSP

AX-FNGP

Tubes

Pupillary distance: 50-75 mm, Inclination angle: 15-35 degrees, Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100 via DSC Zooming Port

  • NI-TT2 Quadrocular Tilting Tube 2, With interlock function
  • NI-TT2-E Motorized Quadrocular Tilting Tube 2, With interlock function

Eyepieces (FN)

CFI 10X (22), CFI 12.5X (16), CFI 15X (14.5), CFI UW 10X (25)

Photodetector

AX-NEU Non-descanned EPI Upright Detector

Nosepieces

  • FN-S2N CFI60 Sliding Nosepiece, Forward-backward sliding type, two positions, DIC prism slider can be attached
  • FN-S2N-2 CFI90 2 Place Sliding Nosepiece*5, Forward-backward sliding type, two positions, DIC prism slider can be attached to the front objective
  • FN-MN-H CFI75 Holder*5, one position, DIC prism slider can be attached
  • FN-MN-H2 CFI90 Holder*3, one position
  • AX-FNTN-H CFI75 Single Tilting Nosepiece*3, *4, *5, one position

Observation methods

Brightfield, Epi-fluorescence, DIC

Power consumption

100W

Weight (approx.)

66 kg (fully motorized fluorescence system, with diascopic illuminator)

66 kg (fully motorized fluorescence system)

Scan head

AX R MP

Type

AX-SHRM AX R MP Scan Head & Controller

Field number (FN)

φ22 mm

Standard image acquisition

Galvano scanner
Pixel size: max. 8192 x 8192 pixels
Scanning speed: max. 240 fps (512 x 16 pixels), 10 fps (512 x 512 pixels)

High-speed image acquisition

Resonant scanner
Pixel size: max. 2048 x 2048 pixels
Scanning speed: max. 720 fps (2048 x 16 pixels for 2K, 1024 x 16 pixels for 1K), 30 fps (2048 x 512 pixels for 2K, 1024 x 512 pixels for 1K)

Scan mode

Line scanning, bi-direction scanning and averaging

Simultaneous acquisition

Max. 5 channels (including a diascopic detector channel)

IR laser wavelength range

700-1080 nm (1080 system), 820-1300 nm (1300 system)

Dichroic mirror

Position: 6

Pinhole

6-153 µm variable

Zoom

1–1000X continuously variable

Input/output port

2 laser input ports
2 signal output ports

Laser for multiphoton microscopy

AX R MP

Single 1080 system

Mai Tai HP/eHP DeepSee, Chameleon Vision II, Axon 920*6,Chameleon Discovery LX

Dual 1080 system

Chameleon Vision II + Axon 920*6, Axon 920*6 + Axon 1064*7, Chameleon Discovery LX + Axon 920*6

Single 1300 system

InSight X3 + InSight X3+, Chameleon Discovery NX

Dual 1300 system

InSight X3 Dual Option, InSight X3+ Dual Option, Chameleon Discovery NX, Chameleon Discovery NX + Axon 920*6

Incident optics

700-1080 nm (1080 system), 820-1300 nm (1300 system), auto alignment

Modulation

Method: AOM (Acousto-Optic Modulator) device
Control: power control, ROI exposure control

Laser for confocal microscopy (option)

AX R MP

4-laser unit

405 nm, 488 nm, 561 nm and 640 nm lasers are installed

5-laser unit

405 nm, 488 nm, 561 nm, 594 nm and 640 nm lasers are installed

6-laser unit

405 nm, 445 nm, 488 nm, 515 nm, 561 nm and 640 nm lasers are installed

NDD for multiphoton microscopy

AX R MP

NDD EPI unit AX-NEI (for Ti2-E) and AX-NEU (for AX-FNSP/FNGP)

Detectable wavelength range: 400-650 nm (1080 system), 400-750 nm (1300 system)

Detectors: 2 GaAsP PMTs (4 GaAsP PMTs, or 3 GaAsP PMTs + 1 multi-alkali PMT are possible by adding options)

Visible stimulation/IR imaging (option)

AX R MP

Opti-Microscan (for AX-FNSP)

Stimulation wavelength: 405 nm, 488 nm, 561 nm;
Excitation wavelength for imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system)
Stimulation speed: Max. 1 ms (point stimulation), Max. 20 μs/pixel (ROI stimulation)
Stimulation modes: simultaneous, sequential, manual
Stimulation area: square inscribed within a 22 mm-diameter circle, stimulation ROI: arbitrary pattern, no number limit

Diascopic detector (option)

AX R MP

AX-DUT-MP (for AX-FNSP/Ti2-E)*8

Detectable wavelength range: 400-920 nm
Detector: Multi-alkali PMT

Detector for confocal / multiphoton microscopy (option)

AX R MP

DUX-VB detector unit

Detectable wavelength range: 400-650 nm (with IR laser), 400-750 nm (with visible laser); Detection width: 10 nm to 320 nm
Maximum pixel size: 8192 x 8192 (with galvano scanner)
Wavelength resolution: 5 nm, wavelength range variable in 1 nm steps
Compatible with galvano and resonant scanners

2 or 4 channels (Multi-alkali PMT or GaAsP PMT options)

DUX-ST detector unit*9

Detectable wavelength range: 400-750 nm
2 or 4 channels (Multi-alkali PMT or GaAsP PMT options)

NSPARC Detector Unit

Equipped with SPPC (Single Pixel Photon Counter) array detector
Up to 7 barrier filters can be mounted (Mountable filter: QuadBand446/523/600/677, 452/45, 525/50, 593/46, 700/75)
With galvano scanner: Can be used with X resolution of 64 to 8192 pixels, Y resolution of 2 to 8192 pixels
With resonant scanner: Can be used with X resolution of 256, 512, 1024 and 2048 pixels, Y resolution of 128 to 2048 pixels

Option

AX R MP

Motorized XYZ

Motorized XY stage (for AX-FNSP/FNGP/Ti2-E), High-speed piezo Z stage (for Ti2-E), High-speed piezo objective-positioning system (for AX-FNSP/FNGP)

Nosepiece for AX-FNSP/FNGP

AX-FNTN-H CFI75 single tilting nosepiece*4

Software

AX R MP

Acquisition/analysis

Imaging software (equipped with Denoise.ai noise reduction function): NIS-Elements C or NIS-Elements C-ER

Display/image generation

2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing

Image format

JP2, JPG, TIFF, BMP, GIF, PNG, ND2, JFF, JTF, AVI, ICS/IDS

Application

FRAP, FLIP, FRET(option), photoactivation, 3D time-lapse imaging, multipoint time-lapse imaging, colocalization

Control computer

AX R MP

OS

Windows®10 Pro 64 bit, Microsoft Windows® 11 Pro*10

 

AX R MP

Visible stimulation/IR imaging (option)

Stimulation wavelength: 405 nm, 488 nm, 561 nm
Excitation wavelength for Imaging: 800-1080 nm (1080 system), 820-1080 nm (1300 system)

Compatible microscopes

Dedicated AX-FNSP/AX-GNGP motorized upright microscope system, ECLIPSE Ti2-E motorized inverted microscope

Z step

AX-FNSP/FNGP: 0.02 µm, Ti2-E: 0.02 µm

Recommended installation conditions

Temperature 20 - 25˚C, ± 1˚C, air conditioning at all hours
Humidity 60% RH or less (no condensation)

IR stimulation/ IR Imaging (OPTION)

AX R MP

AX-STM-IR IR stimulation unit*8

Stimulation wavelength: 1030-1070 nm, 900-950 nm
Excitation wavelength for imaging: 750-950 nm, 1030-1070 nm
Scanner: Galvano scanner × 2
Stimulation speed: Max. 0.1 ms (point stimulation)
Stimulation area: square inscribed within a 22 mm-diameter circle
Stimulation ROI: arbitrary pattern, Stimulation number: max. 100, Power setting: max. 3 steps for each ROI

*1 Based on the focus position
*2 Software controlled value
*3 DIC prism slider cannot be attached
*4 FN12, Usable objectives: CFI75 LWD 16X W, CFI75 Apochromat LWD 20XC W, CFI75 Apochromat 25XC W, CFI75 Apochromat 25XC W 1300
*5 Cannot be used with diascopic illumination. The FN-MN-H cannot be used with diascopic illumination only when the 400 μm objective piezo positioner (PI) is attached.
*6 Axon 920 : Axon 920-1、Axon 920-2
*7 Axon 1064 : Axon 1064-1、Axon 1064-3
*8 Cannot be mounted on AX-FNGP
*9 Must be used with a confocal laser.
*10 Windows 10 and Windows 11 are trademarks or registered trademarks of Microsoft Corporation in the United States and other countries.