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Harvard Medical School

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Harvard Medical School

The Nikon Imaging Center at Harvard Medical School (NIC@HMS) is a Cell Biology Department, Systems Biology Department, and Biochemistry & Molecular Pharmacology Department core facility for light microscopy developed in partnership with Nikon Instruments Inc. and Micro Video Instruments Inc. The NIC@HMS also has several corporate contributors including Binder GmbH, CellASIC, Chroma Technology, CYTOO, Hamamatsu Photonics, Lumencor, Molecular Devices, Prior Scientific, Sutter Instrument Company and Tokai Hit Co., Ltd.

The mission of the NIC@HMS is to:

  • Enhance basic research by providing access to state-of-the-art microscopy and imaging equipment
  • Provide training courses on basic and advanced light microscopy techniques for the benefit of HMS and the greater Boston research community
  • Introduce the latest innovations in light microscopy and imaging to the HMS community
  • Serve as a learning center for our corporate partners and contributors
  • Provide a dedicated NIC@HMS director for ongoing technical consultation and support

The NIC@HMS was the first Nikon Imaging Center, established in 2001. Nikon has since established several other Imaging Centers in collaboration with universities and research centers worldwide.

Contacto

NIC Director

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Address

Harvard Medical School
25 Shattuck St
Boston
MA 02115
United States


Systems Available

CSU-X1 Spinning Disk Confocal System

George features a Ti inverted microscope with spinning disk confocal scanner and incubation, great for live cell imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Yokogawa CSU-X1 spinning disk confocal
  • 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
  • Hamamatsu ORCA-Flash4.0 V3 sCMOS camera
  • Lumencor SOLA light engine
  • Sistema de incubación cerrado OkoLab

FRAP/CSU-X1 Spinning Disk Confocal System

Lucille features a spinning disk confocal scanner as well as FRAP device for live cell photoactivation/FRAP experiments.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Yokogawa CSU-X1 spinning disk confocal
  • 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
  • Hamamatsu ORCA-ER CCD camera
  • Hamamatsu ORCA-R2 CCD camera
  • Photonic Instruments MicroPoint laser FRAP/photoactivation unit
  • Lumencor SOLA light engine
  • Sistema de incubación superior de platina Okolab

Sistema N-STORM/TIRF de súper resolución

Tobias is a single molecule imaging system featuring N-STORM for 3D single molecule localization and TIRF.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • N-STORM super-resolution single molecule localization microscopy system
  • Agilent MLC400B 4-line laser unit (405nm, 488nm, 561nm, 647nm)
  • Andor iXon 897 EMCCD camera
  • Hamamatsu ImagEM EMCCD camera
  • Lumencor SOLA light engine
  • NIS-Elements software

Large FOV Slide-Scanning System

Michael is a high-throughput large field of view system configured on a Nikon Ti2 and optimized for processing slides.

Components

  • Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
  • Large format DS-Qi2 monochrome CMOS camera
  • Lumencor SPECTRA X light engine
  • NIS-Elements software

Microinjection System

Annyong is a Nikon TE300 inverted microscope configured for routine imaging applications.

Components

  • TE2000-U inverted microscope
  • Sutter XenoWorks Digital Microinjector
  • Hamamatsu ORCA-ER CCD camera
  • Lumencor SOLA light engine

Live-Cell Inverted Widefield System

George Michael is a short-term live-cell imaging system optimized for fast triggered acquisitions.

Components

  • Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • Hamamatsu ORCA-R2 CCD camera
  • Lumencor SPECTRA X light engine
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

Live-Cell Inverted Widefield System

Buster features a Ti2 inverted microscope with full incubation enclosure for long-term live-cell imaging.

Components

  • Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • Lumencor SPECTRA X light engine
  • Sistema de incubación cerrado OkoLab
  • NIS-Elements software

CSU-X1 Spinning Disk Confocal System

Lucille Two is another spinning disk confocal system for live cell imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Yokogawa CSU-X1 spinning disk confocal
  • 5-line laser unit (445nm, 488nm, 515nm, 568nm, 642nm)
  • Lumencor SOLA light engine
  • Sistema de incubación cerrado OkoLab

Live-Cell Inverted Widefield System

Gob features a full enclosure type incubation system on an inverted microscope for long-term live-cell imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Hamamatsu ORCA-ER CCD camera
  • Lumencor SOLA light engine
  • Sistema de incubación cerrado OkoLab

Inverted Widefield Microscope System

Components

  • Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
  • Hamamatsu ORCA-Flash4.0 LT sCMOS camera
  • DS-Fi1c color CCD camera
  • Lumencor SOLA light engine
  • NIS-Elements software

A1Rsi Resonant Scanning Confocal System

Maeby is a fast live imaging system featuring an A1Rsi resonant scanning confocal system with spectral detector and FRAP/photoactivation.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1Rsi resonant scanning confocal system
  • 6-line laser unit (405nm, 457nm, 488nm, 514nm, 561nm, 640nm)
  • A1-DUS 32-channel spectral detector
  • Lumencor SOLA light engine
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

Live-Cell TIRF System

Lindsay is a TIRF imaging system optimized for fast live cell imaging.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • Iluminador TIRF motorizado
  • 6-line laser unit (404nm, 446nm, 491nm, 515nm, 561nm, 642nm)
  • Hamamatsu ImagEM EMCCD camera
  • Sistema de incubación cerrado OkoLab
  • NIS-Elements software