Centro de excelencia
University of Michigan
The Microscopy Core provides access to advanced microscopes as well as the expertise of dedicated imaging scientists. We also provide custom image analysis, comprehensive sample preparation for electron microscopy, tissue clearing, and expansion microscopy.
Contacto
CofE Director
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Address
109 Zina Pitcher DriveAnn Arbor, MI 48105
Website
Systems Available
N-SIM/A1R HD Super-Resolution & Confocal System
This system combines super-resolution using the principle of structured illumination and high-speed resonant scanning confocal on a single microscope, making it easy to optimize for resolution or speed with a variety of samples.
Components
- Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
- N-SIM super-resolution structured illumination microscopy system
- A1R HD high-definition resonant scanning confocal system
- LUN-V 6-line laser unit (405nm, 445nm, 488nm, 515nm, 561nm, 647nm)
- DU4G GaAsP 4-channel confocal detector
- DUV-B 2-channel spectral GaAsP detector
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Lumencor SPECTRA X light engine
- Platina piezoeléctrica Mad City Labs serie Nano-Z del eje Z
- Tokai Hit stage-top incubation system
- NIS-Elements software
TIRF/FRAP/CSU-X1 Spinning Disk Confocal System
This flexible live cell imaging system is configured on a Ti2-E inverted microscope and features TIRF, spinning disk confocal, and a DMD for patterned photostimulation.
Components
- Microscopio invertido Ti2-E con sistema Perfect Focus 4 (PFS4)
- Yokogawa CSU-X1 spinning disk confocal
- H-TIRF motorized illuminator for laser TIRF imaging
- Mightex Polygon400 DMD for patterned illumination (Lumencor AURA II light engine)
- LUNF 3-line laser unit (488nm, 561nm, 640nm) for TIRF imaging
- LUNF 4-line laser unit (405nm, 488nm, 561nm, 640nm) for CSU-X1
- Hamamatsu W-VIEW GEMINI-2C image splitting optics
- 2x Andor iXon 888 EMCCD camera
- Platina piezoeléctrica Mad City Labs serie Nano-Z del eje Z
- Tokai Hit stage-top incubation system
- NIS-Elements software
This A1 point scanning confocal system is configured on a Ti-E inverted microscope and well suited to a variety of both live and fixed cell imaging applications.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- LU4A 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- DU4G GaAsP 4-channel confocal detector
- Tokai Hit stage-top incubation system
- NIS-Elements software
This A1 point scanning confocal system is configured on a Ti-E inverted microscope and well suited to a variety of both live and fixed cell imaging applications.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- LU4A 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- DU4 4-channel confocal detector
- NIS-Elements software
A1si Point Scanning Confocal System
This A1 point scanning confocal system is configured on a Ti-E inverted microscope and also features a spectral detector for linear unmixing of signals from multiple emitters.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- A1si point scanning confocal system
- A1-DUS 32-channel spectral detector
- LU4A 4-line laser unit (405nm, 488nm, 561nm, 647nm)
- DU4 4-channel confocal detector
- NIS-Elements software