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Centro de excelencia

Florida Atlantic University

The FAU Nikon Center of Excellence, a partnership with the FAU Brain institute, seeks to provide scientists and trainees across the university’s six campuses with access to high-performance, cellular imaging tools to advance the study of fundamental biological mechanisms and their contributions to human disorders.

Through our Nikon partnership, the FAU community and its community partners gain access to Nikon’s technical expertise and advanced training support on existing imaging platforms as well as opportunities to explore leading-edge Nikon imaging technologies at the earliest possible stages of development. Installations on the Jupiter, FL campus include multiple confocal platforms with enhanced resolution capability, automated X-Y-Z image capture, and TIRF technology, affording high resolution analysis of single molecules in cellular sub-compartments and large field image capture and reconstruction.

A complete list of available systems can be found on the FAU website link below.

Contacto

CofE Director

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CofE Manager

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Address

Florida Atlantic University
5353 Parkside Dr.
Jupiter, FL 33458


Systems Available

TIRF/A1R HD Resonant Scanning Confocal System

This A1R HD resonant scanning confocal system is ready for the most demanding live cell and tissue imaging applications, featuring stage-top incubation, fast Z piezo stage, and spectral detector for advanced multicolor imaging applications.

Components

  • Ti-E inverted microscope with Perfect Focus System (PFS)
  • A1R HD high-definition resonant scanning confocal system
  • A1-DUS 32-channel spectral detector
  • Motorized TIRF Illuminator Unit
  • 6-line laser unit (408nm, 455nm, 488nm, 514nm, 561nm, 640nm)
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • DU4G GaAsP 4-channel confocal detector
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software

C1si Point Scanning Confocal System

This C1si is a point-scanning confocal system featuring spectral detector for linear unmixing of multiple fluorophores with overlapping spectra and configured on an upright microscope.

Components

  • Eclipse 90i upright microscope
  • C1si point scanning confocal system
  • C1-SHS 32-channel spectral detector
  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
  • DU3 3-channel confocal detector
  • NIS-Elements software

A1R MP Resonant Scanning Multiphoton System

This fast multiphoton system is based on a A1R MP resonant scanning multiphoton on FN1 upright microscope and is ideal for intravital imaging applications.

Components

  • FN1 upright microscope
  • A1R MP resonant scanning multiphoton system
  • Coherent Chameleon Ultra II Ti:S laser unit (tunable 680-1080nm)
  • Nosepiece-mounted Z-axis objective piezo
  • Episcopic non-descanned detectors (NDDs)
  • Prior ZDeck Platform System
  • NIS-Elements software

N-SIM E/A1R HD Super-Resolution & Confocal System

This correlative confocal and super-resoluiton system features both N-SIM E structured illumination and A1R HD resonant scanning confocal, giving users the flexibility to prioritize speed or resolution.

Components

  • Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
  • N-SIM E super-resolution structured illumination microscopy system
  • A1R HD high-definition resonant scanning confocal system
  • DU4G GaAsP 4-channel confocal detector
  • LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm) for confocal
  • LUN-3 3-line laser unit (488nm, 561nm, 640nm) for super-resolution
  • Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
  • Mad City Labs Nano-Z series Z-axis piezo stage
  • Tokai Hit stage-top incubation system
  • NIS-Elements software