Pósters
Multi-omics Spatial Analysis of Colon Cancer Tissue Reveals Emergence of an Immunosuppressive Tumor Maintenance System
junio 2025
Using a combination of modalities such as sequential multiplex immunofluorescence (mIF) and spatial transcriptomics (STx) we investigated spatial distribution, phenotype, function, and gene expression profile within the colon cancer and healthy colonx tissue. Immunosuppressive cells, Treg and M2 macrophages were in the TME. Presence of other immune cells was not sufficient to prevent crypt hyperproliferation. Immune cell infiltration into the crypt was prevented by a stromal cell barrier. While immunosuppressive phenotype was observed using antibodies, a strong presence of immunosuppressive genes was not found in transcriptomic study. The latter showed gene expression signatures related to processes of DNA damage, cell death, and hypoxia in epithelial cell regions.