Posters

Köhler Illumination and Phase Contrast Alignment on the Nikon Ti2

January 2018

Köhler illumination is a fundamental technique for ensuring an evenly illuminated field of view for widefield observation using an optical microscope, and also the first step when aligning phase contrast optics on a microscope. This poster provides a step-by-step walkthrough of Köhler illumination and phase contrast alignment.