Nikon Corporation Healthcare Business Unit | Asia, Oceania & Middle East
Artboard 1
en Change Region

Global Site


Polarized Light/Dispersion Microscope

Microscopes for dispersion staining allow for qualitative analysis and identification of asbestos

Polarized light microscopes can measure the properties of asbestos, such as refractive indices, birefringence, retardation, extinction angles, pleochroism and signs of elongation, helping in the identification of asbestos. LV100ND POL/DS is a high-performance, polarized light microscope with accessories that enable dispersion staining observation at up to 400x magnification.

Download ECLIPSE LV100ND POL Brochure (1.19MB)

Key Features


  • The LV100ND POL/DS employs optics with high extinction factors, facilitating detection and observation of high-precision optical properties.
  • The LV100ND POL/DS enables analysis of birefringence, extinction angles, signs of elongation, refractive indices, birefringence magnitudes, and pleochroism of asbestos.
  • Changing the objectives and PH modules allows observation of asbestos dispersion colors corresponding to the refractive indices of the immersion liquid. The LV100ND POL/DS enables both quantitative analysis and dispersion staining observation of asbestos.
  • A fully rotatable circular graduated stage offers trouble-free sample rotation and accurate analysis during polarization observation.


Mixture of rock wool and chrysotile

The cross Nicols, diagonal position

The cross Nicols, extinction position

Extinction angle


The parallel Nicols, cleavage face aligned with the crosshair

The cross Nicols, extinction angle measured

Sign of elongation

Amosite Positive: Subtractive diagonal position

Crocidolite Negative: Additive diagonal position

Refractive index (refraction index of the liquid nD=1.605)

Chrysotile and amosite

The upper part of a specimen is focused

The lower part of a specimen is focused

Retardation (optical path difference) for obtaining birefringence magnitude


Diagonal position

Subtractive diagonal position confirmed (a test plate is inserted)

Analyzer rotation angle measured (Senarmont compensator is inserted)



Parallel to vibration direction of polarizer

Orthogonal to vibration direction of polarizer

Dispersion colors

nD = 1.550, red-violet to blue

nD = 1.680, pink

nD = 1.680, orange to red ocher