Center of Excellence
Cincinnati Children’s Hospital Medical Center
Nikon Instruments USA has partnered with Cincinnati Children’s Hospital Medical Center, to open a Nikon Center of Excellence for advanced biological imaging. The center will combine Nikon’s technical proficiency with the scientific expertise of the researchers at Cincinnati Children’s Research Foundation to further new developments in biomedical sciences that will ultimately improve child health.
This partnership provides CCRF researchers access to state-of-the art imaging equipment and Nikon technical expertise. In return, Nikon gains valuable feedback and insight from talented scientists using advanced research techniques in the diverse areas of research strength found at the CCRF. Our expertise includes phenotypic imaging and analysis of complex biological systems including developmental biology, neurobiology, stem cell biology and cancer biology. We also have a keen focus on developing and adapting new imaging techniques that can be applied to our researcher's areas of expertise.
Contact
CofE Director
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Address
3333 Burnet AvenueCincinnati, Ohio 45229-3026
(513) 636-4425
Systems Available
AX R Inverted Confocal System
The AX R is a recently developed iteration of Nikon’s flagship confocal system, and is capable of 2k resonant scanning in an expanded 25 mm FOV for fast, gentle imaging of sensitive samples.
Components
- 8K galvo / 2K resonant imaging modes
- 8 excitation lines (405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm, 730 nm)
- Tunable spectral detection
- 600 μm high-speed piezo Z stage
- Stagetop incubator
NIS-Elements software
AX R Upright Confocal System
Similar to the inverted AX R configuration, this system is capable of 2k resonant scanning in an expanded 25 mm FOV for fast, gentle imaging of sensitive samples. This system is ideal for electrophysiology and live animal experiments where an upright configuration is needed.
Components
- 8K galvo / 2K resonant imaging modes
- 8 excitation lines (405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, 640 nm, 730 nm)
- Tunable spectral detection
- 450 μm piezo Z stage
- Electrophysiology capable
NIS-Elements software
Yokogawa SoRa Super-Resolution Spinning Disk
Utilizing optical reassignment on a spinning disk system, the SoRa achieves sub-diffraction limited optical resolution while preserving sample health.
Components
- 8K galvo / 2K resonant imaging modes
- 7 excitation lines (405 nm, 445 nm, 488 nm, 515 nm, 561 nm, 594 nm, 640 nm)
- Dual Hamamatsu Fusion BT 95% QE back-thinned SCMOS cameras
- 600 μm high-speed piezo Z stage
- Stagetop incubator
- Galvo photoactivation/photoconversion at 405 nm
NIS-Elements software
N-STORM/TIRF/A1R HD Super-Resolution & Confocal System
Featuring both the N-STORM single molecule localization and A1R HD resonant scanning confocal, this system is ready for applications ranging from super-resolution to fast live-cell confocal or TIRF.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
A1R HD high-definition resonant scanning confocal system
N-STORM super-resolution single molecule localization microscopy system
- LUN-V high-power 6-line laser unit (405nm, 445nm, 488nm, 514nm, 561nm, 647nm)
- DU4G GaAsP 4-channel confocal detector
- Mad City Labs Nano-Z series Z-axis piezo stage
- Andor DU-897 EMCCD camera (STORM, TIRF)
- Tokai Hit stage-top incubation system
- Lumencor SOLA light engine
NIS-Elements software
A1R Confocal System
Go-to A1 point scanning confocal system for both live and fixed cell imaging applications.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- LUN-A 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- DU4G GaAsP 4-channel confocal detector
NIS-Elements software
A1R HD MP Resonant Scanning Multiphoton System
High-definition resonant scanning multiphoton imaging is unlocked with the A1R HD MP system, configured on an FN1 upright microscope.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- A1R HD MP multiphoton system with high-definition resonant scanner
- Coherent Chameleon Ultra II Ti:S laser unit (tunable 680-1080nm)
LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Nosepiece-mounted Z-axis objective piezo
- Episcopic GaAsP non-descanned detectors (NDDs)
- DU4G GaAsP 4-channel confocal detector
NIS-Elements software
ECLIPSE Ni-E Upright Microscope
This Ni-E upright microscope features both color and monochome CCD cameras, supporting a number of epifluorescence and transmitted light applications.
Components
Ni-E upright microscope
- Lumencor SPECTRA X light engine
NIS-Elements software
High-Speed Triggered Widefield Microscope System
This high-speed hardware-triggered widefield system is well-suited to high throughput applications.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- Andor Zyla 4.2 sCMOS camera
- Lumencor SPECTRA X light engine
NIS-Elements software
High-Speed Triggered Widefield Microscope System
This high-speed hardware-triggered system based on the ECLIPSE Ti2-E inverted microscope provides both speed and large field of view for high throughput applications.
Components
Ti2-E inverted microscope with Perfect Focus System 4 (PFS4)
- Andor Zyla 4.2 sCMOS camera
- Lumencor SPECTRA X light engine
- Lumencor LIDA light engine (RGB transmitted illuminator)
NIS-Elements software
Stereomicroscope System
This SMZ1500 stereo microscope also features epifluorescence and a CMOS camera for recording observations.
Components
- SMZ1500 stereo microscope
- Excelitas X-Cite Fluorescence Lamp Illuminator
- SPOT Imaging Insight CMOS camera
Analysis Workstations
Eight offline image analysis workstations with Nikon's NIS-Elements software. NIS-Elements combines automated intelligence to microscopes, cameras, components and peripherals with powerful archiving, analysis, visualization and archiving tools. Its intuitive interface simplifies workflow and speeds up image acquisition times while providing powerful features such as image stitching, object counting and volume views.