The hidden world revealed: in vivo study of Ca2+ dynamics in plants
10 giu 2020
14:30 EDT / 19:30 BST / 20:30 CEST
Alex Costa, Ph.D.
Dipartimento di Bioscienze, Università degli Studi Milano
14:30 - 15:30 CEST
Older technologies (e.g. aequorin) enabled detection of cytosolic Ca2+signatures triggered by different stimuli at low spatial resolutions. Recent developments in microscopy techniques coupled with the use of plants expressing genetically encoded fluorescent Ca2+ sensors are now revealing a hidden world where Ca2+ increases not only occur in individual, stimulated cells but propagates as waves within the plant body. The use of specific targeting sequences or tissue-specific promoters for sensor expression, is also enabling investigation into Ca2+dynamics in different subcellular compartments and in different tissues.
In the webinar, the technical aspects of the imaging tools needed to perform Ca2+ imaging in plants will be presented.