Pre-trained AI-powered label-free detection of cell regions from brightfield images and measurement of fluorescence intensity at the cellular level
ECLIPSE Ji with Smart Experiment software enables seamless operation from image acquisition to analysis and graph creation. Pre-trained Artificial Intelligence (AI) and pre-defined imaging processes automatically optimize image acquisition and analysis condition settings, providing visualized data and EC50 information with simple operation. Fluorescence intensity measurement is used in a wide range of applications, from basic research of cell biology to drug discovery, including quantification of protein and enzyme activity. This application note introduces an example of using the Intensity Measurement module of Smart Experiment to detect cell regions label-free from brightfield images and measure the fluorescence intensity at the cellular level. We also present an example of quantifying the drug’s effect by automatically visualizing the dose-dependent decrease in fluorescence intensity derived from pMAPK and calculating the IC50.