Centre d’excellence
Florida Atlantic University
The FAU Nikon Center of Excellence, a partnership with the FAU Brain institute, seeks to provide scientists and trainees across the university’s six campuses with access to high-performance, cellular imaging tools to advance the study of fundamental biological mechanisms and their contributions to human disorders.
Through our Nikon partnership, the FAU community and its community partners gain access to Nikon’s technical expertise and advanced training support on existing imaging platforms as well as opportunities to explore leading-edge Nikon imaging technologies at the earliest possible stages of development. Installations on the Jupiter, FL campus include multiple confocal platforms with enhanced resolution capability, automated X-Y-Z image capture, and TIRF technology, affording high resolution analysis of single molecules in cellular sub-compartments and large field image capture and reconstruction.
A complete list of available systems can be found on the FAU website link below.
Contactez
CofE Director
email hidden; JavaScript is required
CofE Manager
email hidden; JavaScript is required
Address
Florida Atlantic University5353 Parkside Dr.
Jupiter, FL 33458
Website
Systems Available
TIRF/A1R HD Resonant Scanning Confocal System
This A1R HD resonant scanning confocal system is ready for the most demanding live cell and tissue imaging applications, featuring stage-top incubation, fast Z piezo stage, and spectral detector for advanced multicolor imaging applications.
Components
- Ti-E inverted microscope with Perfect Focus System (PFS)
- A1R HD high-definition resonant scanning confocal system
- A1-DUS 32-channel spectral detector
- Unité d'éclairage TIRF motorisée
- 6-line laser unit (408nm, 455nm, 488nm, 514nm, 561nm, 640nm)
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- DU4G GaAsP 4-channel confocal detector
- Platine Z piezo série Mad City Labs Nano-Z
- Tokai Hit stage-top incubation system
- NIS-Elements software
A1R MP Resonant Scanning Multiphoton System
This fast multiphoton system is based on an A1R MP resonant scanning multiphoton on an ECLIPSE FN1 upright microscope and is ideal for intravital imaging applications.
Components
- FN1 upright microscope
- A1R MP resonant scanning multiphoton system
- Coherent Chameleon Ultra II Ti:S laser unit (tunable 680-1080nm)
- Nosepiece-mounted Z-axis objective piezo
- Episcopic non-descanned detectors (NDDs)
- Prior ZDeck Platform System
- NIS-Elements software
N-SIM E/A1R HD Super-Resolution & Confocal System
This correlative confocal and super-resolution system features both N-SIM E structured illumination and A1R HD resonant scanning confocal, giving users the flexibility to prioritize speed or resolution.
Components
- Microscope inversé Ti2-E proposant un système à mise au point parfaite 4 (PFS4)
- N-SIM E super-resolution structured illumination microscopy system
- A1R HD high-definition resonant scanning confocal system
- DU4G GaAsP 4-channel confocal detector
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm) for confocal
- LUN-3 3-line laser unit (488nm, 561nm, 640nm) for super-resolution
- Hamamatsu ORCA-Flash4.0 V2+ sCMOS camera
- Platine Z piezo série Mad City Labs Nano-Z
- Tokai Hit stage-top incubation system
- NIS-Elements software
A1R HD25 Resonant Scanning Confocal System
This A1R HD25 resonant-scanning confocal system provides fast automated imaging on our motorized ECLIPSE Ni-E upright microscope.
Components
- Ni-E upright microscope
- A1R HD25 high-definition resonant scanning confocal system
- DU4G GaAsP 4-channel confocal detector
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Lumencor SOLA light engine
- NIS-Elements software
Upright A1 Confocal System
This A1 point-scanning confocal system is configured on an ECLIPSE Ni-E upright microscope and is a great option for applications requiring confocal-level optical sectioning.
Components
- Ni-E upright microscope
- DU4 4-channel confocal detector
- LUN-4 4-line laser unit (405nm, 488nm, 561nm, 640nm)
- Lumencor SOLA light engine
- NIS-Elements software
- NIS-Elements Offline Analysis Workstation
Upright A1R Confocal System
This A1R resonant-scanning confocal system on our ECLIPSE FN1 microscope is perfect for in vivo high-speed imaging experiments and correlative electrophysiology work.
Components
- FN1 upright microscope
- DU4G GaAsP 4-channel confocal detector
- LUN-4 4-line laser unit (408nm, 488nm, 561nm, 640nm)
- Lumencor SOLA light engine
- NIS-Elements software