Seminarios web

Super-resolution spinning disk confocal microscopy for life science research


  • Dr. Laurence Pelletier

    Professor, University of Toronto & Lunenfeld-Tanenbaum Research Institute (Sinai Health)

  • Dr. Kenneth Prehoda

    Professor, University of Oregon

  • Dan Collins

    Applications Specialist, Yokogawa Life Innovations

Super-resolution optical microscopies have fundamentally changed the imaging landscape, providing life scientists with a suite of new research tools. However, such techniques can be difficult to implement, necessitating different compromises that can ultimately limit their applicability. Optical pixel reassignment provides a method for super-resolution that is implemented in the context of a traditional spinning disk confocal microscope. In this webcast, we will explore the concept of optical pixel reassignment and see how it has been applied in cell and developmental biology research. In one example, Dr. Prehoda will describe how live imaging of neural stem cells using both traditional and super-resolution imaging revealed how the plasma membrane plays a role in neurogenesis. In another example, Dr. Pelletier will discuss how the use of “smart” automated low- to super-resolution imaging pipelines can allow us to unravel the mysteries of centrosome biogenesis and function and how the molecular mechanisms underlying their function can go awry in disease states.

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