Fluorescent Filter Cubes

High-performance/high-quality fluorescence filter cubes for research that demands precise signal detection.

Nikon offers a wide range of fluorescence filter cubes with high fluorescence acquisition efficiency to support imaging of a large variety of fluorophores and fluorescent proteins. The large product range includes longpass filter cubes for observation of single-stained samples and bandpass filter cubes suitable for detecting specific wavelengths from multiple-stained samples and unmixing.

Wavelengths with an extremely sharp rising edge characteristic.
High transmittance from 93% to 97% (both excitation and barrier filters).
High fluorescence acquisition efficiency, enabling reduced excitation intensity and sample fading.
The HQ series filters, made of glass with high dimensional accuracy, eliminate the possibility of image shifts when superimposing multi-color images and ensure accurate imaging even in the case of fluorophores with small Stokes shifts.

Longpass filter cubes

Filter Cube	Excitation	Spectral profile	Filter diameter	Excitation filter	Dichroic mirror	Barrier filter
UV-2A	UV	graph	25 mm	355/50	400	410
UV-1A	UV	graph	25 mm	365/10	400	390
V-2A	V	graph	25 mm	400/40	430	440
BV-2A	BV	graph	25 mm	420/40	455	460
B-2A	B	graph	25 mm	470/40	505	510
G-2A	G	graph	25 mm	535/50	575	580

Bandpass filter cubes

Standard series

Filter Cube	Spectral profile	Filter diameter	Excitation filter	Dichroic mirror	Barrier filter
DAPI	graph	25 mm	375/28	415	460/60
GFP-B	graph	25 mm	470/40	500	535/50
FITC	graph	25 mm	480/30	505	535/45
TRITC	graph	25 mm	540/25	565	605/55
Texas Red	graph	25 mm	560/40	595	630/60

HQ series

Filter Cube	Spectral profile	Filter diameter	Excitation filter	Dichroic mirror	Barrier filter
DAPI-U HQ	graph	25 mm	395/25	425	460/50
CFP HQ	graph	25 mm	436/20	455	480/40
GFP HQ	graph	25 mm	470/40	495	525/50
FITC HQ	graph	25 mm	480/40	510	535/50
YFP HQ	graph	25 mm	500/20	515	535/30
Cy3 HQ	graph	25 mm	535/40	565	590/40
mCherry HQ	graph	25 mm	570/40	600	645/75
Cy5 HQ	graph	25 mm	620/60	660	700/75

HQ series(for large FOV imaging with Ti2 microscope)

Filter Cube	Spectral profile	Filter diameter	Excitation filter	Dichroic mirror	Barrier filter
DAPI-U HQ	graph	32 mm	395/25	425	460/50
CFP HQ	graph	32 mm	436/20	455	480/40
GFP HQ	graph	32 mm	470/40	495	525/50
FITC HQ	graph	32 mm	480/40	510	535/50
YFP HQ	graph	32 mm	500/20	515	535/30
Cy3 HQ	graph	32 mm	535/40	565	590/40
mCherry HQ	graph	32 mm	570/40	600	645/75
Cy5 HQ	graph	32 mm	620/60	660	700/75

LED Series (Ts2, Nikon LED light sources)

Filter Cube	Spectral profile	Filter diameter	Excitation filter	Dichroic mirror	Barrier filter
C-LED385*	graph	25mm	390/38	420	475/90
C-LED455	graph	25mm	448/23	465	472
C-LED470*	graph	25mm	470/40	500	534/55
C-LED505	graph	25mm	496/29	518	543/37
C-LED525*	graph	25mm	525/50	560	597/58
C-LED560	graph	25mm	550/50	600	630/75
C-LED590	graph	25mm	561/75	610	652/65
C-LED625*	graph	25mm	621/58	660	706/73

*suitable for D-LEDI Fluorescence LED Illumination System

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