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Nikon Corporation (Nikon) has developed the AX NIR with NSPARC, a super-resolution confocal microscope system capable of high-precision observations in the near-infrared (NIR) spectrum. Whereas most NIR imaging systems are limited by the decreased resolving power of NIR light, the NSPARC provides an approximate two-fold improvement in resolution, yielding a stunning level of clarity for observations in both the NIR and visible spectra.

The result is a significantly improved capacity for multiplexed observation of the detailed localization and dynamics of different biomolecules, supporting studies of cellular processes, including the elucidation of disease mechanisms in drug discovery research. Compared to traditional visible wavelength imaging, NIR imaging is better suited for deeper observations in 3D models with higher levels of physiological relevance, such as tissue and organ-on-chip. Sales of this system will commence on April 30, 2025, through Nikon Solutions Corporation.

The key advancement made by the AX NIR with NSPARC is extending the usable spectrum for super-resolution imaging from a conventional excitation wavelength range of 405 nm–640 nm to 405 nm–785 nm, well into the NIR spectrum. This provides more flexibility in avoiding fluorescence crosstalk between different imaging channels, while also realizing improved resolution. Consequently, the accuracy and reproducibility of simultaneous visualization of multiple molecules are improved, enabling a more precise understanding of biological processes at the molecular level within cells.

The mechanisms underlying the onset and progression of diseases such as cancer and neurodegenerative disorders are increasingly being revealed to involve complex interactions among a variety of biomolecules. Understanding these mechanisms has become a critical challenge in the fields of pathology and drug discovery research. Achieving personalized medicine requires a particularly detailed understanding of the molecular-level changes occurring within individual patients. The AX NIR with NSPARC system expands the observable wavelength range into the near-infrared spectrum, enabling an increase in the number of different molecular species whose interactions can be observed simultaneously, while significantly improving resolution in every imaging channel.

For existing users of the AX / AX R with NSPARC systems, equivalent functionality can be implemented through software updates and the addition of excitation lasers. This ensures that current users can also adapt to the latest research needs.

1. High-precision visualization of molecular interactions through super-resolution multiplex imaging

AX NIR with NSPARC supports long-wavelength excitation of near-infrared fluorescent dyes, expanding the available palette for labeling different molecules for super-resolution observation of their intracellular interactions. This enables research and development departments in biotech and pharmaceutical companies to simultaneously observe multiple target molecules involved in the mechanism of action of novel drug candidate compounds, as well as to perform comprehensive analyses of intracellular signaling pathways to elucidate drug resistance mechanisms. Furthermore, in academic research institutions, it can be utilized for applications such as studying the interactions between multiple therapeutic target molecules in cancer cells and analyzing the accumulation of abnormal protein due to neurodegenerative disease.

2. High-reproducibility data acquisition with reduced crosstalk

This update enables the use of long-wavelength near-infrared fluorescent dyes, significantly reducing crosstalk (the overlapping fluorescence signal from spectrally similar dyes) between different fluorophores during observation of a multi-labeled specimen. This ensures more accurate and reproducible data collection, enhancing the reliability of research outcomes and improving decision-making confidence in drug discovery. It is broadly useful for studying intracellular molecular dynamics in life science research, and particularly effective for the simultaneous observation of multiple pathways in the elucidation of drug efficacy and pharmacology.

The development of AX NIR with NSPARC marks an important step forward for Nikon’s healthcare business. This product aims to contribute especially to the elucidation of complex disease mechanisms, such as those seen in cancer and neurodegenerative disorders. By leveraging multiplex imaging technology using near-infrared probes, we have improved the accuracy and reproducibility of simultaneous observation of multiple molecules, which has been challenging until now.

In drug discovery research, there is an increasing demand for a more detailed molecular-level understanding to realize personalized medicine. We are confident that our product will serve as an advanced solution to meet this demand and contribute to breakthroughs in healthcare and drug discovery research.

Nikon will continue to contribute to the advancement of healthcare and the improvement of people's quality of life through the development of innovative solutions centered around optical technology.